Maintenance of daphnia carinata cultures for use in toxicity testing

Zalizniak, L and Nugegoda, D 2004, 'Maintenance of daphnia carinata cultures for use in toxicity testing', Australasian Journal of Ecotoxicology, vol. 10, no. 1, pp. 65-69.


Document type: Journal Article
Collection: Journal Articles

Title Maintenance of daphnia carinata cultures for use in toxicity testing
Author(s) Zalizniak, L
Nugegoda, D
Year 2004
Journal name Australasian Journal of Ecotoxicology
Volume number 10
Issue number 1
Start page 65
End page 69
Total pages 5
Publisher Australasian Society for Ecotoxicology
Abstract A growing concern for use of local species for toxicity testing leads to the need to create toxicity data banks for these species. In order to have healthy animals to conduct the tests, procedures for maintenance of stock cultures of these species should be developed. The most widespread daphnid in Australia, Daphnia carinata, is considered to be one of the most suitable for toxicity testing of contaminants entering Australian freshwaters. However very little data are available on the culture requirements of the species. In this study different types of food were tested: Chlorella vulgaris cultured in two different media - Keating and Tamiya, Chlorella pyrenoidosa cultured in the same two media, and a suspension of trout pellets. Intrinsic rates of natural increase of individual cultures of D. carinata were determined from "life tables". The best food from among those tested were C. pyrenoidosa cultured in either Keating or Tamiya medium. Two different procedures of individual cultures are proposed for the maintenance of D. carinata for use in toxicity testing, based on tests conducted using different culture volumes
Subject Environmental Impact Assessment
Keyword(s) Daphnia carinata
culture requirements
toxicity testing
DOI - identifier 10.1016/j.ecoenv.2005.03.015
Copyright notice Copyright © 2005 Elsevier
ISSN 1323-3475
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