Investigation of PKC isoform-specific translocation and targeting of the current of the late afterhyperpolarizing potential of myenteric AH neurons

van Nguyen, T, Poole, D, Harvey, J, Stebbing, M and Furness, J 2005, 'Investigation of PKC isoform-specific translocation and targeting of the current of the late afterhyperpolarizing potential of myenteric AH neurons', European Journal of Neuroscience, vol. 21, no. 4, pp. 905-913.


Document type: Journal Article
Collection: Journal Articles

Title Investigation of PKC isoform-specific translocation and targeting of the current of the late afterhyperpolarizing potential of myenteric AH neurons
Author(s) van Nguyen, T
Poole, D
Harvey, J
Stebbing, M
Furness, J
Year 2005
Journal name European Journal of Neuroscience
Volume number 21
Issue number 4
Start page 905
End page 913
Total pages 9
Publisher Blackwell Publishing
Abstract AH neurons in the enteric nervous system play an essential role in initiating intestinal reflexes and factors that control AH neuron excitability therefore influence the state of the digestive system. Prominent afterhyperpolarizations that follow action potentials in these neurons strongly affect their excitability. In the present work, we have investigated the regulation of the afterhyperpolarizing current (IAHP) by protein kinase C (PKC). Electrophysiological responses and protein translocation were investigated in AH neurons of freshly dissected preparations of myenteric ganglia from the guinea-pig ileum, The activator of conventional and novel PKCs, phorbol dibutyrate, but not the activator of novel PKCs, ingenol, blocked the IAHP. Phorbol dibutyrate had no effect on the hyperpolarization- activated current (Ih) or on the A current (IA). Stimulation of synaptic inputs to the neurons also reduced the IAHP, and had no effect on Ih or IA. Phorbol dibutyrate also reduced a background outward current that was present after the IAHP current had been blocked by clotrimazole. Both phorbol dibutyrate and ingenol caused translocation of the novel PKC, PKCepsilon, in these neurons. Only phorbol dibutyrate caused translocation of PKCgamma, a conventional PKC. The studies thus indicate that the activation of PKC by phorbol esters and by nerve stimulation affects AH neurons in a similar way, and that PKC activation targets both the IAHP and another background K+ current. The IAHP is targeted by a conventional PKC, suggested to be PKCgamma, as this is the only conventional PKC that is prominent in AH neurons.
Subject Autonomic Nervous System
DOI - identifier 10.1111/j.1460-9568.2005.03931.x
ISSN 0953-816X
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