Validation of folate in a convenient yeast assay suited for identification of inhibitors of Alzheimer's amyloid-beta aggregation

macreadie, I, Lotfi-Miri, M, Mohotti, S, Shapira, D, Bennett, L and Varghese, J 2008, 'Validation of folate in a convenient yeast assay suited for identification of inhibitors of Alzheimer's amyloid-beta aggregation', Journal of Alzheimer's Disease, vol. 15, no. 3, pp. 391-396.


Document type: Journal Article
Collection: Journal Articles

Title Validation of folate in a convenient yeast assay suited for identification of inhibitors of Alzheimer's amyloid-beta aggregation
Author(s) macreadie, I
Lotfi-Miri, M
Mohotti, S
Shapira, D
Bennett, L
Varghese, J
Year 2008
Journal name Journal of Alzheimer's Disease
Volume number 15
Issue number 3
Start page 391
End page 396
Total pages 6
Publisher IOS Press
Abstract Previously in the search for chemopreventatives for Alzheimer's disease (AD), microbial cells with amyloid-beta (A beta) protein fusions have been used to screen for compounds that reduce the aggregation, misfolding or oligomerization of A beta. In the current study, such a system has been used to look at the effect of folate, whose deficiency has been associated with AD. A folate-deficient yeast strain producing A beta fused to green fluorescent protein (GFP) that spontaneously misfolds/aggregates and suppresses green fluorescence was used to examine the effects of folinic acid on A beta-GFP fluorescence. At levels that did not affect growth or plasmid stability, increasing levels of folinic acid led to increasing green fluorescence, suggesting folate can assist with preventing A beta-misfolding/aggregation. This result supports a protective role for folate and suggests that yeast assays may provide significant new approaches for testing of AD chemopreventatives.
Keyword(s) aggregation
alzheimer's disease chemo preventatives
amyloid-b
flow cytometry
folate
high throughput screening
oligomerization
saccharomyces cerevisiae
yeast assays
ISSN 1387-2877
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