Identification of a parathyroid hormone in the fish Fugu rubripes

Danks, J, Ho, P, Notini, A, Katsis, F, Hoffmann, P, Kemp, B, Martin, T and Zajac, J 2003, 'Identification of a parathyroid hormone in the fish Fugu rubripes', Journal of Bone and Mineral Research, vol. 18, no. 7, pp. 1326-1331.


Document type: Journal Article
Collection: Journal Articles

Title Identification of a parathyroid hormone in the fish Fugu rubripes
Author(s) Danks, J
Ho, P
Notini, A
Katsis, F
Hoffmann, P
Kemp, B
Martin, T
Zajac, J
Year 2003
Journal name Journal of Bone and Mineral Research
Volume number 18
Issue number 7
Start page 1326
End page 1331
Total pages 6
Publisher American Society for Bone and Mineral Research
Abstract A PTH gene has been isolated from the fish Fugu rubripes. The encoded protein of 80 amino acid has the lowest homology with any of the PTH family members. Fugu PTH(1-34) had 5-fold lower potency than human PTH(1-34) in a mammalian cell system. Introduction: Parathyroid hormone (PTH) is the major hypercalcemic hormone in higher vertebrates. Fish lack parathyroid glands, but there have numerous attempts to identify and isolate PTH from fish. Materials and Methods: Polymerase chain reaction (PCR) was performed with primers based on preliminary data from the Joint Genome Institute database. PCR amplification was performed on genomic DNA isolated from Fugu rubripes. PCR products were purified and DNA was sequenced. All sequence was confirmed from more than one independently amplified PCR product. Multiple sequence alignments were carried out, and the percentage of identities and similarities were calculated. An unrooted phylogenetic tree, using all the known PTH and PTH-related protein (PTHrP) amino acid sequences, was determined. Synthetic peptides were tested in a biological assay that measured cyclic adenosine 3',5' -monophosphate formation in UMR106.1 cells. Rabbit polyclonal antisera specific for N-terminal human PTHrP and one rabbit polyclonal antiserum specific for N terminus hPTH were used to test the cross-reactivity with fPTH(1-34) in immunoblots.
Subject Fish Physiology and Genetics
Keyword(s) amino acid sequence
amino terminal sequence
animal cell
article
biosynthesis
calcium homeostasis
controlled study
cross reaction
DNA isolation
DNA purification
DNA sequence
fish
fugu rubripes
gene duplication
gene isolation
hormone action
hormone determination
hormone structure
immunoblotting
molecular evolution
nonhuman
phylogenetic tree
polymerase chain reaction
sequence alignment
sequence analysis
sequence homology
vertebrate
DOI - identifier 10.1359/jbmr.2003.18.7.1326
ISSN 0884-0431
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