Effect of short-term phytoestrogen treatment in male rats on nitric oxide-mediated responses of carotid and cerebral arteries: Comparison with 17Beta-estradiol

Sobey, C, Weiler, J, Boujaoude, M and Woodman, O 2004, 'Effect of short-term phytoestrogen treatment in male rats on nitric oxide-mediated responses of carotid and cerebral arteries: Comparison with 17Beta-estradiol', Journal of Pharmacology and Experimental Therapeutics, vol. 310, no. 1, pp. 135-140.


Document type: Journal Article
Collection: Journal Articles

Title Effect of short-term phytoestrogen treatment in male rats on nitric oxide-mediated responses of carotid and cerebral arteries: Comparison with 17Beta-estradiol
Author(s) Sobey, C
Weiler, J
Boujaoude, M
Woodman, O
Year 2004
Journal name Journal of Pharmacology and Experimental Therapeutics
Volume number 310
Issue number 1
Start page 135
End page 140
Total pages 6
Publisher American Society for Pharmacology and Experimental Therapeutics
Abstract The use of estrogen for protection against vascular dysfunction is limited due to its effects on the reproductive system, particularly in males. We postulated that daidzein, an isoflavone with estrogen-like effects on the systemic vasculature but not the reproductive system, might enhance nitric oxide (NO)-mediated cerebral vasodilatation. Male rats were administered vehicle, 17beta-estradiol (0.1 mg/kg s.c.), or daidzein (0.2 mg/kg s.c.) daily for 7 days. Basal and acetylcholine-stimulated NO release was assessed in vitro via carotid arterial rings or in vivo by measuring changes in basilar artery diameter. Levels of protein expression of endothelial NO synthase (eNOS), caveolin-1, and calmodulin were assessed in carotid arteries using Western analysis. Plasma NO levels were doubled by daidzein or 17beta-estradiol. NO production and endothelium-dependent contrac-tion in response to the NOS inhibitor NG-nitro-L-arginine (L-NNA; 100 mu M) was enhanced by 50 to 100% in carotid arteries from rats treated with daidzein or 17beta-estradiol. Acetylcholine-induced relaxation was selectively enhanced in carotid arteries from rats treated with daidzein. Similarly, constrictor responses of the basilar artery to L-NNA in vivo were selectively augmented by -100% by 17beta-estradiol treatment and tended to be -50% greater in daidzein-treated rats. Expression of caveolin-1 was decreased, and calmodulin was increased, in vessels from daidzein- or 17beta-estradiol-treated rats. eNOS expression was unaffected by the treatments. These data suggest that short-term administration of daidzein or 17beta-estradiol modulates cerebral artery reactivity in males by enhancing synthesis and release of endothelium-derived NO. Isoflavone therapy may therefore be a feasible approach to protect against cerebrovascular disease and stroke.
Subject Basic Pharmacology
DOI - identifier 10.1124/jpet.103.063255
ISSN 0022-3565
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