Annexin-1 peptide Anx-12-26 protects adult rat cardiac myocytes from cellular injury induced by simulated ischaemia

Ritchie, R, Gordon, J, Woodman, O, Cao, A and Dusting, G 2005, 'Annexin-1 peptide Anx-12-26 protects adult rat cardiac myocytes from cellular injury induced by simulated ischaemia', British Journal of Pharmacology, vol. 145, pp. 495-502.


Document type: Journal Article
Collection: Journal Articles

Title Annexin-1 peptide Anx-12-26 protects adult rat cardiac myocytes from cellular injury induced by simulated ischaemia
Author(s) Ritchie, R
Gordon, J
Woodman, O
Cao, A
Dusting, G
Year 2005
Journal name British Journal of Pharmacology
Volume number 145
Start page 495
End page 502
Total pages 8
Publisher John Wiley and Sons Ltd.
Abstract The anti-inflammatory properties of annexin-1 peptides have been largely ascribed to their powerful antineutrophil actions in vivo. We have recently reported that the N-terminal fragment of annexin-1, Anx-12-26, preserves contractile function of cardiac muscle in vitro. The aim of the present study was to determine if Anx-12-26 elicits protective actions specifically on the cardiac myocyte (in the absence of neutrophils), using a model of metabolic inhibition to simulate ischaemia. 2 Metabolic inhibition of cardiac myocytes (4 h incubation at 37ºC in HEPES-containing buffer supplemented with 2-deoxy-D-glucose, D,L-lactic acid and pH adjusted to 6.5) followed by 2.5 h recovery in normal medium markedly increased creatine kinase (CK) and lactate dehydrogenase (LDH) levels by 179 ± 39 and 26 ± 7IU L-1 (both n = 40, P < 0.001), respectively. However, cellular injury was significantly decreased when Anx-12-26 (0.3 mu;M) was present during metabolic inhibition, CK by 74 ± 10% and LDH by 71 ± 6% (both n = 31, P < 0.001), respectively. 3 Boc 2 (10 mu M), a nonselective formyl peptide receptor antagonist, present during metabolic inhibition, abolished the cardioprotective effect of Anx-12-26. 4 Addition of chelerythrine (10 mu M), 5-hydroxydecanoate (500 mu M) or SB202190 (1 mu M) during metabolic inhibition also abolished Anx-12-26- induced cardioprotection. 5 Cellular injury induced by metabolic inhibition was also largely prevented when myocytes were incubated with Anx-12-26 for 5 min with 10 min recovery prior to the insult, or when Anx-12-26 was present only during the recovery period following drug-free metabolic inhibition. 6 In conclusion, the annexin-1 peptide Anx-12-26 potently prevents cardiac myocyte injury induced by metabolic inhibition, an action that was dependent at least in part on the activation of the formyl peptide receptor family of G-protein-coupled receptors, protein kinase C, p38 mitogen-activated protein kinase and ATP-sensitive potassium channels.
Subject Cardiology (incl. Cardiovascular Diseases)
DOI - identifier 10.1038/sj.bjp.0706211
ISSN 0007-1188
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