Determination of adefovir in human plasma by liquid chromatography/tandem mass spectrometry: Application to a pharmacokinetic study

Bi, H, Zhong, G, Zhou, S, Chen, X and Huang, M 2005, 'Determination of adefovir in human plasma by liquid chromatography/tandem mass spectrometry: Application to a pharmacokinetic study', Rapid Communications in Mass Spectrometry, vol. 19, no. 20, pp. 2911-2917.


Document type: Journal Article
Collection: Journal Articles

Title Determination of adefovir in human plasma by liquid chromatography/tandem mass spectrometry: Application to a pharmacokinetic study
Author(s) Bi, H
Zhong, G
Zhou, S
Chen, X
Huang, M
Year 2005
Journal name Rapid Communications in Mass Spectrometry
Volume number 19
Issue number 20
Start page 2911
End page 2917
Total pages 7
Publisher John Wiley and Sons Ltd.
Abstract A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated to determine the concentrations of adefovir [9-(2-phosphonylmethoxyethyl)adenine, PMEA] in human plasma. After one-step protein precipitation of plasma samples by methanol, adefovir was analyzed by LC/MS/MS using positive electrospray ionization. Chromatography was performed on a C18 column. The extraction recoveries of adefovir were found to be 85.1-89.3%. Adefovir was stable under routine laboratory conditions. A minimal matrix effect resulting in a slight ionization enhancement of adefovir (<10.9%) was observed, which did not markedly affect the behavior of the calibrations curves and accuracy and precision data. The method had a chromatographic run time of 7.8 min and a linear calibration curve over the concentration range 1.5-90 ng/ mL for adefovir. The lower limit of quantification of the method was 1.5 ng/mL. The intra- and inter-day precision was less than 8.4%. These results indicated that this LC/MS/MS method has high selectivity and efficiency, and acceptable accuracy, precision and sensitivity. The validated LC/MS/MS method has been successfully used in a pharmacokinetic study in healthy volunteers treated with oral adefovir dipivoxil at 10 and 20 mg.
Subject Basic Pharmacology
DOI - identifier 10.1002/rcm.2141
ISSN 0951-4198
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