Penetratin tandemly linked to a CTL peptide induces anti-tumour T-cell responses via a cross-presentation pathway

Pouniotis, D, Apostolopoulos, V and Pietersz, G 2006, 'Penetratin tandemly linked to a CTL peptide induces anti-tumour T-cell responses via a cross-presentation pathway', Immunology, vol. 117, no. 3, pp. 329-339.


Document type: Journal Article
Collection: Journal Articles

Title Penetratin tandemly linked to a CTL peptide induces anti-tumour T-cell responses via a cross-presentation pathway
Author(s) Pouniotis, D
Apostolopoulos, V
Pietersz, G
Year 2006
Journal name Immunology
Volume number 117
Issue number 3
Start page 329
End page 339
Total pages 11
Publisher Wiley-Blackwell Publishing Ltd.
Abstract Recently there has been increasing evidence to suggest that membrane translocating peptides enter cells by a receptor-dependent pathway. There have been some studies on the mechanism of major histocompatibility complex (MHC) class I presentation of membrane translocating peptides incorporating cytotoxic T lymphocyte epitopes. However, these have been on different cell lines and only a limited number of inhibitors of the antigen presentation pathway were used. Herein, we demonstrate a comprehensive study utilizing a full spectrum of inhibitors to various pathways of MHC class I to elucidate the mechanism of the membrane translocating peptide, penetratin from Antennapedia (Int). It is clear that Int, RQIKIWFQNRRMKWKK when tandemly linked to a cytotoxic T lymphocyte peptide of ovalbumin, SIINFEKL (IntSIIN) is endocytosed via phagocytosis or macropinocytosis by dendritic cells in an ATP-dependent manner and is processed by a proteasome- and tapasin-independent pathway for presentation and loading to MHC class I molecules. In addition, the majority of antigen is taken up by negatively charged receptors. IntSIIN activates T cells in vitro and in vivo and protects mice against challenge with an ovalbumin-expressing tumour.
Subject Immunology not elsewhere classified
DOI - identifier 10.1111/j.1365-2567.2005.02304.x
Copyright notice © 2005 Blackwell Publishing Ltd.
ISSN 0019-2805
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