Regulation of HSL serine phosphorylation in skeletal muscle and adipose tissue

Watt, M, Holmes, A, Pinnamaneni, S, Garnham, A, Steinberg, G, Kemp, B and Febbraio, M 2006, 'Regulation of HSL serine phosphorylation in skeletal muscle and adipose tissue', American Journal of Physiology: Endocrinology and Metabolism, vol. 290, pp. E500-E508.

Document type: Journal Article
Collection: Journal Articles

Title Regulation of HSL serine phosphorylation in skeletal muscle and adipose tissue
Author(s) Watt, M
Holmes, A
Pinnamaneni, S
Garnham, A
Steinberg, G
Kemp, B
Febbraio, M
Year 2006
Journal name American Journal of Physiology: Endocrinology and Metabolism
Volume number 290
Start page E500
End page E508
Total pages 9
Publisher American Physiological Society
Abstract Hormone-sensitive lipase (HSL) is important for the degradation of triacylglycerol in adipose and muscle tissue, but the tissue-specific regulation of this enzyme is not fully understood. We investigated the effects of adrenergic stimulation and AMPK activation in vitro and in circumstances where AMPK activity and catecholamines are physiologically elevated in humans in vivo (during physical exercise) on HSL activity and phosphorylation at Ser563 and Ser660, the PKA regulatory sites, and Ser565, the AMPK regulatory site. In human experiments, skeletal muscle, subcutaneous adipose and venous blood samples were obtained before, at 15 and 90 min during, and 120 min after exercise. Skeletal muscle HSL activity was increased by 80% at 15 min compared with rest and returned to resting rates at the cessation of and 120 min after exercise. Consistent with changes in plasma epinephrine, skeletal muscle HSL Ser563 and Ser660 phosphorylation were increased by 27% at 15 min (P < 0.05), remained elevated at 90 min, and returned to preexercise values postexercise. Skeletal muscle HSL Ser565 phosphorylation and AMPK signaling were increased at 90 min during, and after, exercise. Phosphorylation of adipose tissue HSL paralleled changes in skeletal muscle in vivo, except HSL Ser660 was elevated 80% in adipose compared with 35% in skeletal muscle during exercise. Studies in L6 myotubes and 3T3-L1 adipocytes revealed important tissue differences in the regulation of HSL
Subject Cell Metabolism
Exercise Physiology
Keyword(s) Endocrinology & Metabolism
DOI - identifier 10.1152/ajpendo.00361.2005
Copyright notice © 2006 American Physiological Society.
ISSN 0193-1849
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