Effect of ultraviolet radiation on the expression of pp38MAPK and furin in human keratinocyte-derived cell lines

Huynh, T, Chan, K and Piva, T 2009, 'Effect of ultraviolet radiation on the expression of pp38MAPK and furin in human keratinocyte-derived cell lines', Photodermatology Photoimmunology and Photomedicine, vol. 25, no. 1, pp. 20-29.


Document type: Journal Article
Collection: Journal Articles

Title Effect of ultraviolet radiation on the expression of pp38MAPK and furin in human keratinocyte-derived cell lines
Author(s) Huynh, T
Chan, K
Piva, T
Year 2009
Journal name Photodermatology Photoimmunology and Photomedicine
Volume number 25
Issue number 1
Start page 20
End page 29
Total pages 10
Publisher Blackwell Publishing
Abstract Background: Ultraviolet radiation (UVR) is known to induce the activation of stress-inflammation signal transduction pathways, and to induce the activity of many proteases in skin cells. It is unknown whether the activation of proteases such as furin is related to changes in the phosphorylation status of p38MAPK. Methods: The effect of UVR on immortalized keratinocyte (HaCaT) and squamous cell carcinoma (Colo16) cells was investigated with respect to cell survival, phosphorylation of p38MAPK, and the proprotein convertase, furin. The cells were exposed to either a low or a high dose of UVA and/or UVB and the viability was monitored over 48h, along with changes in the intracellular expression of p38MAPK and furin. Results: Low-dose UVA (2 kJ/m2) and/or UVB (0.2 kJ/m2) radiation had no effect on cell viability, except in UVA-irradiated Colo16 cells. High UVA (20 kJ/m2) caused a loss of cell viability in HaCaT cells, but not in Colo16 cells. The opposite effect was seen in cells exposed to a high UVB dose (2 kJ/m2).
Subject Cancer Cell Biology
Keyword(s) Furin
Keratinocytes
pp38MAPK
squamous cell carcinoma
ultraviolet (UV) radiation
DOI - identifier 10.1111/j.1600-0781.2009.00395.x
Copyright notice © 2009 The Authors Journal compilation © 2009 Blackwell Munksgaard.
ISSN 0905-4383
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