Oxidized and reduced mannan mediated MUC1 DNA immunization induce effective anti-tumor responses

Tang, C, Sheng, K, Pouniotis, D, Esparon, S, Son, H, KIM, C, Pietersz, G and Apostolopoulos, V 2008, 'Oxidized and reduced mannan mediated MUC1 DNA immunization induce effective anti-tumor responses', Vaccine, vol. 23, no. 31, pp. 3827-3834.


Document type: Journal Article
Collection: Journal Articles

Title Oxidized and reduced mannan mediated MUC1 DNA immunization induce effective anti-tumor responses
Author(s) Tang, C
Sheng, K
Pouniotis, D
Esparon, S
Son, H
KIM, C
Pietersz, G
Apostolopoulos, V
Year 2008
Journal name Vaccine
Volume number 23
Issue number 31
Start page 3827
End page 3834
Total pages 8
Publisher Elsevier Ltd
Abstract DNA immunization is an attractive form of vaccination, which has shown promising results only in small animal models. There is a need to develop efficient gene delivery systems. We previously demonstrated that oxidized (OM) and reduced mannan (RM) complexed to ovalbumin DNA via poly-l-lysine (PLL), were able to generate potent immune responses in mice. Herein, we further investigated the suitability of OMPLL and RMPLL as carriers for mucin 1 (MUC1) DNA vaccination for cancer immunotherapy. Studies presented here showed that immune responses in C57BL/6 mice induced by OMPLL-MUC1 DNA and RMPLL-MUC1 DNA immunization were more immunogenic compared to MUC1 DNA alone. Moreover, tumor protection was evident at a dose as low as 0.5 ?g. In addition, strong T cell responses were induced in HLA-A2 transgenic and human MUC1 transgenic mice. These results demonstrate the potential of OM and RM as efficient non-viral gene delivery carriers for DNA vaccines for use in cancer immunotherapy.
Subject Pharmacology and Pharmaceutical Sciences not elsewhere classified
Keyword(s) DNA
Vaccines
Gene delivery
Mannan
MUC1
DOI - identifier 10.1016/j.vaccine.2008.05.008
Copyright notice © 2008 Elsevier Ltd. All rights reserved.
ISSN 0264-410X
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