Mannan-mediated gene delivery for cancer immunotherapy

Tang, C, Lodding, J, Minigo, G, Pouniotis, D, Plebanski, M, Scholzen, A, McKenzie, I, Pietersz, G and Apostolopoulos, V 2007, 'Mannan-mediated gene delivery for cancer immunotherapy', Immunology, vol. 120, no. 3, pp. 325-335.

Document type: Journal Article
Collection: Journal Articles

Title Mannan-mediated gene delivery for cancer immunotherapy
Author(s) Tang, C
Lodding, J
Minigo, G
Pouniotis, D
Plebanski, M
Scholzen, A
McKenzie, I
Pietersz, G
Apostolopoulos, V
Year 2007
Journal name Immunology
Volume number 120
Issue number 3
Start page 325
End page 335
Total pages 11
Publisher Wiley-Blackwell Publishing Ltd.
Abstract Recent years have seen a resurgence in interest in the development of efficient non-viral delivery systems for DNA vaccines and gene therapy. We have previously used oxidized and reduced mannan as carriers for protein delivery to antigen-presenting cells by targeting the receptors that bind mannose, resulting in efficient induction of cellular responses. In the present study, oxidized mannan and reduced mannan were used as receptor-mediated gene transfer ligands for cancer immunotherapy. In vivo studies in C57BL/6 mice showed that injection of DNA encoding ovalbumin (OVA) complexed to oxidized or reduced mannan-poly-L-lysine induced CD8 and CD4 T-cell responses as well as antibody responses leading to protection of mice from OVA+ tumours. Both oxidized and reduced mannan delivery was superior to DNA alone or DNA-poly-L-lysine. These studies demonstrate the potential of oxidized and reduced mannan for efficient receptor-mediated gene delivery in vivo, particularly as DNA vaccines for cancer immunotherapy.
Subject Immunology not elsewhere classified
Keyword(s) DNA vaccine
mannose receptor
gene therapy
oxidized mannan
DOI - identifier 10.1111/j.1365-2567.2006.02506.x
ISSN 0019-2805
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