Oxidative stress and increased eNOS and NADPH oxidase expression in mouse microvessel endothelial cells

Ding, H, Aljofan, M and Triggle, C 2007, 'Oxidative stress and increased eNOS and NADPH oxidase expression in mouse microvessel endothelial cells', Journal of Cellular Physiology, vol. 212, pp. 682-689.


Document type: Journal Article
Collection: Journal Articles

Title Oxidative stress and increased eNOS and NADPH oxidase expression in mouse microvessel endothelial cells
Author(s) Ding, H
Aljofan, M
Triggle, C
Year 2007
Journal name Journal of Cellular Physiology
Volume number 212
Start page 682
End page 689
Total pages 8
Publisher Wiley-Liss
Abstract Elevated oxidative stress plays a key role in diabetes-associated vascular disease. In this study, we tested the hypothesis that high glucose-induced oxidative stress was associated with changes in the expression of NADPH oxidase, superoxide dismutase (SOD) and endothelial nitric oxide synthase (eNOS). Oxidative stress was assessed in cell cultures of mouse microvessel endothelial cells (MMECs) by fluorescence labelling with dihydroethidium, lucigenin-enhanced chemiluminescence and determining NADPH oxidase subunit and eNOS expression with real-time polymerase chain reaction protocol and Western blotting. Oxidative stress and expression of the NADPH oxidase subunit, p22phox, were both increased, SOD I and 3 expression lowered and eNCS significantly elevated in MMECs treated with 40 mM glucose for 72 h compared to low glucose medium. Oxidative stress, p22phox mRNA, eNOS mRNA, and protein were lowered by concurrent incubation with sepiapterin. When eNOS protein expression in endothelial cells was significantly decreased by eNOS siRNA treatment, superoxide generation was significantly higher in the MMECs grown in low glucose, but reduced in those grown in high glucose for 72 h. Thus, exposure of MMECs to high glucose results in increased oxidative stress that is associated with increased eNOS and NADPH oxidase subunit expression, notably p22phox, and decreased expression of SOD and 3.
Subject Cell Physiology
Keyword(s) Small Mesenteric-Arteries
Nox Isoform Expression
Vascular Smooth-Muscle
Protein-Kinase-C
Superoxide-Dismutase
Nad(P)H Oxidase
Nitric-Oxide
Gp91(Phox) Homologs
Angiotensin-Ii
Diabetic Mice
DOI - identifier 10.1002/jcp.21063
Copyright notice © 2007 Wiley-Liss
ISSN 0021-9541
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 60 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 52 times in Scopus Article | Citations
Altmetric details:
Access Statistics: 177 Abstract Views  -  Detailed Statistics
Created: Fri, 07 Jan 2011, 09:11:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us