Novel anti-inflammatory omega-3 PUFAs from the New Zealand green-lipped mussel, Perna canaliculus

Treschow, A, Hodges, L, Wright, P, Wynne, P, Kalafatis, N and Macrides, T 2007, 'Novel anti-inflammatory omega-3 PUFAs from the New Zealand green-lipped mussel, Perna canaliculus', Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, vol. 147, no. 4, pp. 645-656.


Document type: Journal Article
Collection: Journal Articles

Title Novel anti-inflammatory omega-3 PUFAs from the New Zealand green-lipped mussel, Perna canaliculus
Author(s) Treschow, A
Hodges, L
Wright, P
Wynne, P
Kalafatis, N
Macrides, T
Year 2007
Journal name Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume number 147
Issue number 4
Start page 645
End page 656
Total pages 12
Publisher Elsevier Science
Abstract The present study has identified in the marine mollusc, Perna canaliculus, an homologous series of novel omega 3 polyunsaturated fatty acids (omega-3 PUFA) with significant anti-inflammatory (Al) activity. The free fatty acid (FFA) class was isolated from a supercritical-CO2 lipid extract of the tartaric acid-stabilised freeze-dried mussel powder by normal phase chromatography, followed by reversed-phase high performance liquid chromatography (RP-HPLC). The RP-HPLC involved separation based on carbon numbers, followed by argentation-HPLC (Ag-HPLC) of the methyl esters based on degree of unsaturation. Identification of the FFA components was performed using gas chromatography (GC) with flame ionisation detection, and individual structures were assigned by GC-mass spectroscopy (GC-MS). Inhibition of leukotriene production by stimulated human neutrophils was used as an in vitro screening method to test the Al activity of the purified PUFAs. A structurally related family of omega-3 PUFAs was identified in the most bioactive fractions, which included C 18:4, C 19:4, C20:4, and C21:5 PUFA. The C20:4 was the predominant PUFA in the extract, and was a structural isomer of arachidonic acid (AA). The novel compounds may be biologically significant as Al agents, as a result of their in vitro inhibition of lipoxygenase products of the AA pathway.
Keyword(s) Extracts
DOI - identifier 10.1016/j.cbpb.2007.04.004
Copyright notice Copyright © 2007 Elsevier Inc. All rights reserved.
ISSN 1096-4959
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