Gonadotropins regulate rat testicular tight junctions in vivo

McCabe, M, Tarulli, G, Meachem, S, Robertson, D, Smooker, P and Stanton, P 2010, 'Gonadotropins regulate rat testicular tight junctions in vivo', Endocrinology, vol. 151, no. 6, pp. 2911-2922.


Document type: Journal Article
Collection: Journal Articles

Title Gonadotropins regulate rat testicular tight junctions in vivo
Author(s) McCabe, M
Tarulli, G
Meachem, S
Robertson, D
Smooker, P
Stanton, P
Year 2010
Journal name Endocrinology
Volume number 151
Issue number 6
Start page 2911
End page 2922
Total pages 12
Publisher The Endocrine Society
Abstract Sertoli cell tight junctions (TJs) are an essential component of the blood-testis barrier required for spermatogenesis; however, the role of gonadotropins in their maintenance is unknown. This study aimed to investigate the effect of gonadotropin suppression and short-term replacement on TJ function and TJ protein (occludin and claudin-11) expression and localization, in an adult rat model in vivo. Rats (n = 10/group) received the GnRH antagonist, acyline, for 7 wk to suppress gonadotropins. Three groups then received for 7 d: 1) human recombinant FSH, 2) human chorionic gonadotropin (hCG) and rat FSH antibody (to study testicular androgen stimulation alone), and 3) hCG alone (to study testicular androgen and pituitary FSH production). TJ proteins were assessed by real-time PCR, Western blot analysis, and immunohistochemistry, whereas TJ function was assessed with a biotin permeation tracer. Acyline treatment significantly reduced testis weights, serum androgens, LH and FSH, and adluminal germ cells (pachytene spermatocyte, round and elongating spermatids). In contrast to controls, acyline induced seminiferous tubule permeability to biotin, loss of tubule lumens, and loss of occludin, but redistribution of claudin-11, immunostaining. Short-termhormonereplacement stimulated significant recoveries in adluminal germ cell numbers. In hCG±FSH antibody-treated rats, occludin and claudin-11 protein relocalized at the TJ, but such relocalization was minimal with FSH alone. Tubule lumens also reappeared, but most tubules remained permeable to biotin tracer, despite the presence of occludin. It is concluded that gonadotropins maintain Sertoli cell TJs in the adult rat via a mechanism that includes the localization of occludin and claudin-11 at functional TJs.
Keyword(s) acyline
androgen
biotin
chorionic gonadotropin
claudin 11
follitropin
gonadotropin
gonadotropin antagonist
hormone antibody
immunoglobulin
luteinizing hormone
occludin
recombinant follitropin
tracer
unclassified drug
Cldn11 protein
rat
gonadorelin
membrane protein
nerve protein
oligopeptide
adult
androgen blood level
animal cell
animal experiment
animal model
article
cell count
controlled study
follitropin blood level
germ cell
hormone substitution
hormone synthesis
human
immunohistochemistry
in vivo study
luteinizing hormone blood level
male
nonhuman
pachytene
permeability
priority journal
protein depletion
protein expression
protein function
protein localization
rat
real time polymerase chain reaction
seminiferous tubule
Sertoli cell
spermatid
spermatocyte
spermatogenesis
testis
testis weight
tight junction
Western blotting
animal
blood
drug antagonism
drug effect
metabolism
polymerase chain reaction
spermatogonium
Sprague Dawley rat
Androgens
Animals
Blotting
Western
Chorionic Gonadotropin
Follicle Stimulating Hormone
Gonadotropin-Releasing Hormone
Gonadotropins
Immunohistochemistry
Luteinizing Hormone
Male
Membrane Proteins
Nerve Tissue Proteins
Oligopeptides
Polymerase Chain Reaction
Rats
Rats
Sprague-Dawley
Spermatids
Spermatocytes
Spermatogonia
Testis
Tight Junctions
DOI - identifier 10.1210/en.2009-1278
Copyright notice Copyright © 2010 by The Endocrine Society.
ISSN 0013-7227
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Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
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