Oxidative stress-induced insulin resistance in skeletal muscle cells is ameliorated by gamma-tocopherol treatment

Singh, I, Carey, A, Watson, N, Febbraio, M and Hawley, J 2008, 'Oxidative stress-induced insulin resistance in skeletal muscle cells is ameliorated by gamma-tocopherol treatment', European Journal of Nutrition, vol. 47, no. 7, pp. 387-392.


Document type: Journal Article
Collection: Journal Articles

Title Oxidative stress-induced insulin resistance in skeletal muscle cells is ameliorated by gamma-tocopherol treatment
Author(s) Singh, I
Carey, A
Watson, N
Febbraio, M
Hawley, J
Year 2008
Journal name European Journal of Nutrition
Volume number 47
Issue number 7
Start page 387
End page 392
Total pages 6
Publisher Springer
Abstract Background Oxidative stress-induced reactive oxygen species are associated with the clinical manifestation of insulin resistance. Evidence suggests that antioxidant treatment may reduce this incidence. Aim of the study This study determined whether glucose oxidase (GO)-induced insulin resistance in cultured skeletal muscle cells could be ameliorated by pre-treatment with gamma-tocopherol (GT). Methods Insulin sensitivity in L6 myotubes was assessed by 2-deoxy-d-[3H]-glucose uptake. The phosphorylation of distal insulin signaling proteins Akt and the Akt substrate AS160 were determined by western blot. Results One hour treatment with 100 mU/ml GO decreased insulin-stimulated glucose uptake (P < 0.001). Pre-treatment with GT either partially (100 μM) or completely (200 μM) restored insulin-stimulated glucose uptake in cells after GO-induced insulin resistance. GO-induced oxidative stress did not impair insulin stimulated phosphorylation of Akt or AS160, but 200 μM GT increased insulin-stimulated phosphorylation of these key signaling proteins (P < 0.05). Conclusions High-dose (200 μM) GT treatment ameliorated oxidative stress-induced insulin resistance in cultured rat L6 skeletal muscle cells.
Subject Exercise Physiology
Keyword(s) glucose oxidase - L6 myotubes - Akt - AS160 - glucose transport - antioxidants
DOI - identifier 10.1007/s00394-008-0739-2
Copyright notice © Steinkopff Verlag 2008
ISSN 1436-6207
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 25 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 22 times in Scopus Article | Citations
Altmetric details:
Access Statistics: 168 Abstract Views  -  Detailed Statistics
Created: Fri, 07 Jan 2011, 09:11:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us