Comprehensive two-dimensional gas chromatography - Quadrupole mass spectrometric analysis of drugs

Song, S, Marriott, P and Wynne, P 2004, 'Comprehensive two-dimensional gas chromatography - Quadrupole mass spectrometric analysis of drugs', Journal of Chromatography A, vol. 1058, pp. 223-232.


Document type: Journal Article
Collection: Journal Articles

Title Comprehensive two-dimensional gas chromatography - Quadrupole mass spectrometric analysis of drugs
Author(s) Song, S
Marriott, P
Wynne, P
Year 2004
Journal name Journal of Chromatography A
Volume number 1058
Start page 223
End page 232
Total pages 10
Publisher Elsevier
Abstract The use of comprehensive two-dimensional gas chromatography coupled to quadrupole mass spectrometry (GC × GC-qMS) for drug screening is investigated with 77 underivatised drug standards in methanolic solution. The GC × GC-qMS setup involved a reduced mass scan range of 42-235 u and minimum quadrupole sampling time to achieve quadrupole scanning frequency of 19.36 Hz. Only 26% of the drugs investigated gave fair-to-acceptable library matches with full mass scan range commercial libraries. The creation of a new "truncated" library based on the mass spectra of the drug standards in the applied mass scan range of 42-235 u extends the feasibility of the currently adopted GC × GC-qMS approach to higher molecular weight compounds and is investigated with blank blood spiked with drug standards. With the new library, 75% of the drugs yielded matches of at least 90%. The time-of-flight mass spectrometer (TOFMS) is expected to address the limitations of the present GC × GC-qMS setup and a brief comparison between GC × GC-qMS and GC × GC-TOFMS is also provided in this study.
Subject Separation Science
Keyword(s) Drug screening
multi-dimensional chromatography
mass spectrometry library
DOI - identifier 10.1016/j.chroma.2004.08.087
Copyright notice © 2004 Elsevier B.V. All rights reserved
ISSN 0021-9673
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