A PMMA microfluidic droplet platform for In vitro protein expression using crude E coli S30 extract

Wu, N, Zhu, Y, Brown, S, Oakeshott, J, Peat, T, Surjadi, R, Easton, C, Leech, P and Sexton, B 2009, 'A PMMA microfluidic droplet platform for In vitro protein expression using crude E coli S30 extract', Lab On a Chip: microfluidic and nanotechnologies for chemistry, biology, and bioengineering, vol. 9, no. 23, pp. 3391-3398.


Document type: Journal Article
Collection: Journal Articles

Title A PMMA microfluidic droplet platform for In vitro protein expression using crude E coli S30 extract
Author(s) Wu, N
Zhu, Y
Brown, S
Oakeshott, J
Peat, T
Surjadi, R
Easton, C
Leech, P
Sexton, B
Year 2009
Journal name Lab On a Chip: microfluidic and nanotechnologies for chemistry, biology, and bioengineering
Volume number 9
Issue number 23
Start page 3391
End page 3398
Total pages 8
Publisher Royal Society of Chemistry
Abstract Droplet based microfluidics are promising new tools for biological and chemical assays. In this paper, a high throughput and high sensitivity microfluidic droplet platform is described for in vitro protein expression using crude Escherichia coli S30 extract. A flow-focusing polymethylmethacrylate (PMMA) microchip was designed and integrated with different functions involving droplet generation, storage, separation and detection. The material used for the chip is superior to the previously tested polydimethylsiloxane (PDMS) due to its mechanical and chemical properties. Droplet formation characteristics such as size and generation rate are investigated systematically. The effect of surfactants Abil EM90 and Span80 in the oil phase on droplet formation and optical detection is also studied. The performance of the system is demonstrated by the high throughput and stable droplet generation and ultralow detection limit. The robustness of the system is also demonstrated by the successful synthesis of a green fluorescent protein (GFP) using E. coli S30 extract as a source of RNA translation reagents.
Subject Electrical and Electronic Engineering not elsewhere classified
DOI - identifier 10.1039/b911581a
Copyright notice © 2009 The Royal Society of Chemistry.
ISSN 1473-0197
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