Antioxidant activity contributes to flavonol cardioprotection during reperfusion of rat hearts

Qin, C, Williams, S and Woodman, O 2011, 'Antioxidant activity contributes to flavonol cardioprotection during reperfusion of rat hearts', Free Radical Biology & Medicine, vol. 51, no. 7, pp. 1437-1444.


Document type: Journal Article
Collection: Journal Articles

Title Antioxidant activity contributes to flavonol cardioprotection during reperfusion of rat hearts
Author(s) Qin, C
Williams, S
Woodman, O
Year 2011
Journal name Free Radical Biology & Medicine
Volume number 51
Issue number 7
Start page 1437
End page 1444
Total pages 8
Publisher Elsevier Inc
Abstract The mechanism of flavonol-induced cardioprotection is unclear. We compared the protective actions of a flavonol that inhibits calcium utilization and has antioxidant activity, 3',4'-dihydroxyflavonol (DiOHF); a flavonol that affects only calcium activity, 4'-OH-3'-OCH3- flavonol (4'-OH-3'-OCH3F); and a water-soluble flavonol with selective antioxidant activity, DiOHF-6-succinamic acid (DiOHF-6-SA), in isolated, perfused rat hearts. Hearts were subjected to global ischemia for 20 min followed by 30 min reperfusion and were treated with vehicle (0.05% DMSO), DiOHF, 4'-OH-3'-OCH3F, or DiOHF-6-SA (all 10 uM, n = 5-8 per group). Flavonols were infused for 10 min before ischemia and during reperfusion. In vehicle-treated hearts, left-ventricular (LV) + dP/dt was reduced by 60% at the end of reperfusion compared to the preischemic level. Lactate dehydrogenase (LDH) release was elevated and endothelial NO synthase (eNOS) expression was lower in vehicle-treated hearts compared to shams. In comparison, DiOHF treatment improved LV function upon reperfusion, decreased LDH, and preserved eNOS expression. The antioxidant DiOHF-6-SA also preserved contractility, reduced LDH, and preserved eNOS expression. In contrast, hearts treated with 4'-OH-3'-OCH3F showed a degree of contractile impairment similar to that of the vehicle group. DiOHF and DiOHF-6-SA also exerted cardioprotection when given only during reperfusion and not when administered only before ischemia. Flavonol-induced cardioprotection relies on antioxidant activity and is mainly exerted during reperfusion.
Subject Cardiology (incl. Cardiovascular Diseases)
Keyword(s) Antioxidant
Cardioprotection
Flavonol
Free radicals
Heart
Ischemia/reperfusion
DOI - identifier 10.1016/j.freeradbiomed.2011.07.003
Copyright notice © 2011 Elsevier Inc.
ISSN 0891-5849
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