Further developments in high temperature HPLC-electrospray mass spectrometry and the introduction of a novel electrophoretic trapping mechanism

Mende, J 2011, Further developments in high temperature HPLC-electrospray mass spectrometry and the introduction of a novel electrophoretic trapping mechanism, Masters by Research, Applied Sciences, RMIT University.


Document type: Thesis
Collection: Theses

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Title Further developments in high temperature HPLC-electrospray mass spectrometry and the introduction of a novel electrophoretic trapping mechanism
Author(s) Mende, J
Year 2011
Abstract This thesis explores various aspects of high temperature HPLC and develops a novel trapping technique in electrophoretic conditions, incorporating a forward pumping action which is a hybrid between HPLC and CE.

Temperature programming and elevated isothermal temperatures have been used in HPLC to demonstrate that it is a viable technique for reducing analysis time and improving sensitivity of analyses. Selectivity changes and improved resolution are shown to be possible by means of altering temperature and in some cases by altering eluent composition. Inverse temperature programming combined with gradient elution is also described.

With an applied electric field, as a trapping device in CE, analytes can be concentrated at an electrode which is placed near the flow stream prior to an analytical HPLC column. Organic modifiers are required to aid retention and it has been shown that non-aqueous CE has been explored recently. Since a pump is not used in conventional CE, a flat flow profile is given instead of a parabolic profile and sensitivity can be increased prior to an analytical HPLC separation. This could be considered as a hybrid technique which combines aspects that are found in both HPLC and CE, using novel focusing methods. This thesis employs micro HPLC concepts, along with micro UV detection and electrospray methods.
Degree Masters by Research
Institution RMIT University
School, Department or Centre Applied Sciences
Keyword(s) HPLC
CE
ESMS
micro-HPLC
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Created: Thu, 22 Mar 2012, 09:26:05 EST by Guy Aron
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