Behaviour of viruses in biosolids

Chellappan, S 2013, Behaviour of viruses in biosolids, Masters by Research, Applied Science, RMIT University.


Document type: Thesis
Collection: Theses

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Title Behaviour of viruses in biosolids
Author(s) Chellappan, S
Year 2013
Abstract Biosolids refers to the stabilized sewage sludge produced in wastewater treatment which is destined for beneficial purposes as a fertilizer and soil conditioner. In addition to nutrients and organic matter, biosolids may contain pathogens including bacteria, viruses, helminths and parasites. The current Victorian guidelines to produce T1 treatment grade biosolids (unrestricted use) require the sludge to be stored for a minimum of three years after the pan drying process to ensure destruction of pathogens. This long term storage is associated with problems such as loss of nutrients in biosolids management costs incurred in maintaining stockpiles of sludge and increased greenhouse gas emissions from the stockpiles. This study evaluates whether microbiological criteria with respect to viruses can be achieved with a shorter treatment time. This data will be provided to regulatory authorities prior to their revision of current Victorian guidelines. In this study, adenovirus was chosen as indicator pathogen for enteric viruses. Primary objectives of this project include: (i) development of standard qPCR method for the detection of adenovirus in sewage sludge, (ii) determining the treatment time required by adenoviruses to achieve prescribed microbiological criteria through laboratory simulation of pan drying and stockpiling processes of sludge collected from both metropolitan and regional wastewater treatment systems (iii) finding the levels of human adenobirus 41 (HAdV 41) in metropolitan sludge at different stages of the treatment process (output from mesophilic anaerobic digester, drying pans of different ages) and regional sludge treatment systems (lagoon sludge) in Victoria. These studies will be used to determine whether adenviruses could serve as better indicator organisms than coliphages in biosolids treatment.
Degree Masters by Research
Institution RMIT University
School, Department or Centre Applied Science
Keyword(s) Biosolids
Adenovirus
Treatment time
T1 grade
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Created: Tue, 03 Sep 2013, 08:27:07 EST by Brett Fenton
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