Anti-proliferative activity of oral anti hyperglycemic agents on human vascular smooth muscle cells: Thiazolidinediones (glitazones) have enhanced activity under high glucose conditions

Little, P, Osman, N, de Dios, S, Cemerlang, N, Ballinger, M and Nigro, J 2007, 'Anti-proliferative activity of oral anti hyperglycemic agents on human vascular smooth muscle cells: Thiazolidinediones (glitazones) have enhanced activity under high glucose conditions', Cardiovascular Diabetology, vol. 6, no. 33, pp. 1-9.


Document type: Journal Article
Collection: Journal Articles

Title Anti-proliferative activity of oral anti hyperglycemic agents on human vascular smooth muscle cells: Thiazolidinediones (glitazones) have enhanced activity under high glucose conditions
Author(s) Little, P
Osman, N
de Dios, S
Cemerlang, N
Ballinger, M
Nigro, J
Year 2007
Journal name Cardiovascular Diabetology
Volume number 6
Issue number 33
Start page 1
End page 9
Total pages 9
Publisher BioMed Central Ltd.
Abstract Background: Inhibition of vascular smooth muscle cell (vSMC) proliferation by oral anti-hyperglycemic agents may have a role to play in the amelioration of vascular disease in diabetes. Thiazolidinediones (TZDs) inhibit vSMC proliferation but it has been reported that they anomalously stimulate [3H]-thymidine incorporation. We investigated three TZDs, two biguanides and two sulfonylureas for their ability of inhibit vSMC proliferation. People with diabetes obviously have fluctuating blood glucose levels thus we determined the effect of media glucose concentration on the inhibitory activity of TZDs in a vSMC preparation that grew considerably more rapidly under high glucose conditions. We further explored the mechanisms by which TZDs increase [3H]-thymidine incorporation. Methods: VSMC proliferation was investigated by [3H]- thymidine incorporation into DNA and cell counting. Activation and inhibition of thymidine kinase utilized short term [3H]- thymidine uptake. Cell cycle events were analyzed by FACS.
Subject Cardiovascular Medicine and Haematology not elsewhere classified
DOI - identifier 10.1186/1475-2840-6-33
Copyright notice © 2007 Little et al; licensee BioMed Central Ltd
ISSN 1475-2840
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