Both alpha-haemolysin determinants contribute to full virulence of uropathogenic Escherichia coli strain 536

Nagy, g, Altenhoefer, A, Knapp, O, Maier, E, Dobrindt, U, Blum-Oehler, G, Benz, R and Emody, L 2006, 'Both alpha-haemolysin determinants contribute to full virulence of uropathogenic Escherichia coli strain 536', Microbes and Infection, vol. 8, no. 8, pp. 2006-2012.


Document type: Journal Article
Collection: Journal Articles

Title Both alpha-haemolysin determinants contribute to full virulence of uropathogenic Escherichia coli strain 536
Author(s) Nagy, g
Altenhoefer, A
Knapp, O
Maier, E
Dobrindt, U
Blum-Oehler, G
Benz, R
Emody, L
Year 2006
Journal name Microbes and Infection
Volume number 8
Issue number 8
Start page 2006
End page 2012
Total pages 7
Publisher Elsevier Masson
Abstract Uropathogenic Escherichia coli strain 536 possesses two intact copies of the ?-haemolysin determinant localised on distinct pathogenicity islands. The coding regions of the two hlyCABD operons are conserved; however, upstream sequences are entirely dissimilar. Consequently, expression of the encoded toxin molecules in vitro is highly different. On the other hand, the contribution of the individual determinants to the strain's virulence is the same. Isogenic mutants lacking individual hly determinants have a similar increase in LD50 value in a mouse model of urinary tract infection. Mouse lung toxicity as well as in vitro assays reveals a significant decrease in acute cytotoxicity of both mutants in comparison to the parent wild-type strain; however, the two hly mutants do not significantly differ from each other in these respects. Single channel recordings show no difference in electrophysiological characteristics of the pores formed by the individual HlyA molecules on synthetic planar lipid membranes. Nor do the paralogues have any target cell preference in an in vitro cytotoxicity assay. Our data suggest that the two hly paralogues encode identical toxin functions; however, due to different regulation of expression, they participate at distinct stages of the infectious process. Interestingly, the unrelated uropathogenic E. coli strain J96 shares the same two hly alleles, suggesting that acquisition of the two paralogues accorded a selective evolutionary advantage.
Subject Microbiology not elsewhere classified
Keyword(s) alpha-Haemolysin
Cytotoxicity
Gene duplication
Pore formation
Uropathogenic Escherichia coli
Virulence regulation
DOI - identifier 10.1016/j.micinf.2006.02.029
Copyright notice © 2006 Elsevier SAS. All rights reserved
ISSN 1286-4579
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