A novel method for simultaneous Enterococcus species identification/typing and van genotyping by high resolution melt analysis

Grando, D, Mayall, B, Wang, J and Ghaly-Derias, S 2012, 'A novel method for simultaneous Enterococcus species identification/typing and van genotyping by high resolution melt analysis', Journal of Microbiological Methods, vol. 90, no. 3, pp. 167-181.


Document type: Journal Article
Collection: Journal Articles

Title A novel method for simultaneous Enterococcus species identification/typing and van genotyping by high resolution melt analysis
Author(s) Grando, D
Mayall, B
Wang, J
Ghaly-Derias, S
Year 2012
Journal name Journal of Microbiological Methods
Volume number 90
Issue number 3
Start page 167
End page 181
Total pages 15
Publisher Elsevier BV
Abstract In order to develop a typing and identification method for van gene containing Enterococcus faecium, two multiplex PCR reactions were developed for use in HRM-PCR (High Resolution Melt-PCR): (i) vanA, vanB, vanC, vanC23 to detect van genes from different Enterococcus species; (ii) ISR (intergenic spacer region between the 16S and 23S rRNA genes) to detect all Enterococcus species and obtain species and isolate specific HRM curves. To test and validate the method three groups of isolates were tested: (i) 1672 Enterococcus species isolates from January 2009 to December 2009; (ii) 71 isolates previously identified and typed by PFGE (pulsed-field gel electrophoresis) and MLST (multi-locus sequence typing); and (iii) 18 of the isolates from (i) for which ISR sequencing was done.
Subject Bacteriology
Infectious Agents
Anaesthesiology
Keyword(s) Enterococcus
genotyping
high resolution melt analysis
identification
typing
vancomycin
DOI - identifier 10.1016/j.mimet.2012.05.002
Copyright notice © 2012 Elsevier B.V.
ISSN 0167-7012
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
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