Cardiac-like flow generator for long-term imaging of endothelial cell responses to circulatory pulsatile flow at microscale

Chen, H, Cornwell, J, Zhang, H, Lim, T, Resurreccion, R, Port, T, Rosengarten, G and Nordon, R 2013, 'Cardiac-like flow generator for long-term imaging of endothelial cell responses to circulatory pulsatile flow at microscale', Lab on a Chip: Miniaturisation for Chemistry, Physics, Biology, Materials Science and Bioengineering, vol. 13, no. 15, pp. 2999-3007.


Document type: Journal Article
Collection: Journal Articles

Title Cardiac-like flow generator for long-term imaging of endothelial cell responses to circulatory pulsatile flow at microscale
Author(s) Chen, H
Cornwell, J
Zhang, H
Lim, T
Resurreccion, R
Port, T
Rosengarten, G
Nordon, R
Year 2013
Journal name Lab on a Chip: Miniaturisation for Chemistry, Physics, Biology, Materials Science and Bioengineering
Volume number 13
Issue number 15
Start page 2999
End page 3007
Total pages 9
Publisher R S C Publications
Abstract In vitro models of circulatory hemodynamics are required to mimic the microcirculation for study of endothelial cell responses to pulsatile shear stress by live cell imaging. This study reports the design, fabrication and characterisation of a microfluidic device that generates cardiac-like flow in a continuous culture system with a circulatory volume of only 2-3 μL. The device mimics a single chamber heart, with the following cardiac phases: (1) closure of the ventricle inlet valve, (2) contraction of the ventricle (systole) followed by opening of the outlet valve and (3) relaxation of the ventricle (diastole) with opening of the inlet valve whilst the outlet valve remains closed. Periodic valve states and ventricular contractions were actuated by microprocessor controlled pneumatics. The time-dependent velocity-field was characterised by micro-particle image velocimetry (µ-PIV). µ-PIV observations were used to help tune electronic timing of valve states and ventricular contractions for synthesis of an arterial pulse waveform to study the effect of pulsatile shear stress on bovine artery endothelial cells (BAECs). BAECs elongated and aligned with the direction of shear stress after 48 h of exposure to a pulsatile waveform with a maximum shear stress of 0.42 Pa. The threshold for BAECs alignment and elongation under steady (non-pulsatile) flow reported by Kadohama et al. (2006) is 0.7-1.4 Pa. These cells respond to transient shear stress because the time average shear stress of the pulse waveform to generate this morphological response was only 0.09 Pa, well below the steady flow threshold. The microfluidic pulse generator can simulate circulatory hemodynamics for live cell imaging of shear-induced signalling pathways.
Subject Renewable Power and Energy Systems Engineering (excl. Solar Cells)
Keyword(s) animal cell
article
bovine artery endothelial cell
diastole
endothelium cell
heart cycle
heart valve
heart ventricle contraction
hemodynamic parameters
microcirculation
microfluidic pulse generator
microfluidics
microprocessor
nanofabrication
nonhuman
particle image velocimetry
priority journal
pulsatile flow generator
pulse wave
shear stress
signal transduction
DOI - identifier 10.1039/c3lc50123j
Copyright notice © The Royal Society of Chemistry 2013
ISSN 1473-0197
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