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A novel technique for the formation of embryoid bodies inside liquid marbles Sarvi, F, Arbatan, T, Chan, P and Shen, W 2013, 'A novel technique for the formation of embryoid bodies inside liquid marbles', RSC Advances, vol. 3, no. 34, pp. 14501-14508. Document type: Journal Article Collection: Journal Articles Attached Files Name Description MIMEType Size n2006045938.pdf Published Version application/pdf 1.00MB Title A novel technique for the formation of embryoid bodies inside liquid marbles Author(s) Sarvi, F Arbatan, T Chan, P Shen, W Year 2013 Journal name RSC Advances Volume number 3 Issue number 34 Start page 14501 End page 14508 Total pages 8 Publisher R S C Publications Abstract The significant and inherent limitations associated with commonly used methods of in vitro embryoid body (EB) formation motivate the development of novel, facile, efficient and reproducible techniques. In this study we report the possibility of using ''liquid marbles'' as facile and efficient micro bioreactors for in vitro EB formation. To exploit liquid marbles as micro biological reactors, embryonic stem cells (ES cells) were inoculated into liquid marbles containing embryonic cell growth medium. Herein we show how the confined internal space of a liquid marble, along with the porous and non-adhesive properties of the highly hydrophobic liquid marble shell, can provide the necessary conditions for the formation of uniform EBs inside liquid marbles. Factors such as the powder particle size, the liquid marble volume and the cell seeding density inside each liquid marble were also investigated to evaluate the effects of varying experimental conditions on the efficiency of EB formation within a liquid marble. Subject Biomaterials DOI - identifier 10.1039/c3ra40364e Copyright notice © The Royal Society of Chemistry 2013 ISSN 2046-2069 Related Links Link Description http://dx.doi.org/10.1039/c3ra40364e Link to Published Version   Versions Version Filter Type Fri, 16 Jan 2015, 08:08:51 EST Tue, 03 Mar 2015, 23:15:24 EST Tue, 19 Jan 2016, 10:29:11 EST Filtered Full Citation counts:   Cited 32 times in Thomson Reuters Web of Science Article | Citations Cited 19 times in Scopus Article | Citations Altmetric details: Access Statistics: 156 Abstract Views, 77 File Downloads  -  Detailed Statistics Created: Thu, 15 Jan 2015, 13:42:00 EST by Catalyst Administrator