A novel technique for the formation of embryoid bodies inside liquid marbles

Sarvi, F, Arbatan, T, Chan, P and Shen, W 2013, 'A novel technique for the formation of embryoid bodies inside liquid marbles', RSC Advances, vol. 3, no. 34, pp. 14501-14508.


Document type: Journal Article
Collection: Journal Articles

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Title A novel technique for the formation of embryoid bodies inside liquid marbles
Author(s) Sarvi, F
Arbatan, T
Chan, P
Shen, W
Year 2013
Journal name RSC Advances
Volume number 3
Issue number 34
Start page 14501
End page 14508
Total pages 8
Publisher R S C Publications
Abstract The significant and inherent limitations associated with commonly used methods of in vitro embryoid body (EB) formation motivate the development of novel, facile, efficient and reproducible techniques. In this study we report the possibility of using ''liquid marbles'' as facile and efficient micro bioreactors for in vitro EB formation. To exploit liquid marbles as micro biological reactors, embryonic stem cells (ES cells) were inoculated into liquid marbles containing embryonic cell growth medium. Herein we show how the confined internal space of a liquid marble, along with the porous and non-adhesive properties of the highly hydrophobic liquid marble shell, can provide the necessary conditions for the formation of uniform EBs inside liquid marbles. Factors such as the powder particle size, the liquid marble volume and the cell seeding density inside each liquid marble were also investigated to evaluate the effects of varying experimental conditions on the efficiency of EB formation within a liquid marble.
Subject Biomaterials
DOI - identifier 10.1039/c3ra40364e
Copyright notice © The Royal Society of Chemistry 2013
ISSN 2046-2069
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