Cathepsin S causes inflammatory pain via biased agonism of PAR2 and TRPV4

Zhao, P, Lieu, T, Barlow, N, Metcalf, M, Veldhuis, N, Jensen, D, Kocan, M, Sostegni, S, Haerteis, S, Baraznenok, V, Henderson, I, Lindstrom, E, Guerrero-Alba, R, Valdez-Morales, E, Liedtke, W, McIntyre, P, Vanner, S, Korbmacher, C and Bunnett, N 2014, 'Cathepsin S causes inflammatory pain via biased agonism of PAR2 and TRPV4', Journal of Biological Chemistry, vol. 289, no. 39, pp. 27215-27234.


Document type: Journal Article
Collection: Journal Articles

Title Cathepsin S causes inflammatory pain via biased agonism of PAR2 and TRPV4
Author(s) Zhao, P
Lieu, T
Barlow, N
Metcalf, M
Veldhuis, N
Jensen, D
Kocan, M
Sostegni, S
Haerteis, S
Baraznenok, V
Henderson, I
Lindstrom, E
Guerrero-Alba, R
Valdez-Morales, E
Liedtke, W
McIntyre, P
Vanner, S
Korbmacher, C
Bunnett, N
Year 2014
Journal name Journal of Biological Chemistry
Volume number 289
Issue number 39
Start page 27215
End page 27234
Total pages 20
Publisher American Society for Biochemistry and Molecular Biology
Abstract Serine proteases such as trypsin and mast cell tryptase cleave protease-activated receptor-2 (PAR2) at R36/S37 and reveal a tethered ligand that excites nociceptors, causing neurogenic inflammation and pain. Whether proteases that cleave PAR2 at distinct sites induce inflammation and pain by a biased signaling mechanism is unknown. We observed that cathepsin S (Cat-S) cleaved PAR2 at E56/T57. In cell lines and nociceptive neurons, Cat-S and a decapeptide mimicking the Cat-S-revealed tethered ligand stimulated PAR2 coupling to Gs and formation of cAMP. Unlike trypsin, Cat-S did not mobilize intracellular Ca2+, activate ERK1/2, recruit ß-arrestins, or induce PAR2 endocytosis. Cat-S caused PAR2-dependent activation of transient receptor potential vanilloid 4 (TRPV4) in Xenopus oocytes, HEK cells and neurons, and stimulated neuronal hyperexcitability by adenylyl cyclase and protein kinase A-dependent mechanisms. Cat-S caused inflammation and hyperalgesia in mice that was attenuated by PAR2 or TRPV4 deletion and adenylyl cyclase inhibition. Cat-S and PAR2 antagonists suppressed formalin-induced inflammation and pain. Our results reveal a novel mechanism of Cat-S-activation of PAR2, and expand the role of PAR2 as a mediator of protease-driven inflammatory pain.
Subject Signal Transduction
Receptors and Membrane Biology
Keyword(s) G protein-coupled receptor (GPCR)
inflammation
pain
protease
protease-activated receptors
transient receptor potential channels (TRP channels
DOI - identifier 10.1074/jbc.M114.599712
Copyright notice © 2014 The American Society for Biochemistry and Molecular Biology, Inc
ISSN 1083-351X
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 75 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 47 times in Scopus Article | Citations
Altmetric details:
Access Statistics: 152 Abstract Views  -  Detailed Statistics
Created: Thu, 15 Jan 2015, 13:42:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us