A potential protein-based vaccine for influenza H5N1 from the recombinant HA1 domain of avian influenza A/H5N1 expressed in Pichia pastoris

Nguyen, T, Van, T, Lin, Y, Van, T, Bui, K, Le, Q, Do, T, Hong Le, T, Vo, V, Truong, V, Smooker, P, Coloe, P and Truong, N 2014, 'A potential protein-based vaccine for influenza H5N1 from the recombinant HA1 domain of avian influenza A/H5N1 expressed in Pichia pastoris', Future Virology, vol. 9, no. 12, pp. 1019-1031.


Document type: Journal Article
Collection: Journal Articles

Title A potential protein-based vaccine for influenza H5N1 from the recombinant HA1 domain of avian influenza A/H5N1 expressed in Pichia pastoris
Author(s) Nguyen, T
Van, T
Lin, Y
Van, T
Bui, K
Le, Q
Do, T
Hong Le, T
Vo, V
Truong, V
Smooker, P
Coloe, P
Truong, N
Year 2014
Journal name Future Virology
Volume number 9
Issue number 12
Start page 1019
End page 1031
Total pages 13
Publisher Future Medicine
Abstract The HA1 genes from influenza A strains A/Puerto Rico/8/1934 H1N1 (A/PR/8/34) and A/Hatay/2004 H5N1 were each cloned in Pichia pastoris vectors in the correct reading frame with the yeast α-factor secretion signal and the C-terminus His-tag, resulting in simple, fast purification of expressed H1HA1 and H5HA1 protein from the culture medium. Mice vaccinated with the purified proteins showed robust T cell, anti-HA1 IgG responses and developed a high antibody response for hemagglutination inhibition (HI) at titer 7.6 log2. Chickens vaccinated with a dose of 200 µg of H5HA1 mixed with either Montanide or Freund's adjuvants gave HI values of up to 7 log2 at the third week comparable with a licensed inactivated H5N1 vaccine.
Subject Virology
Keyword(s) avian influenza virus
H5N1
HA1
Hemagglutinin
Pichia pastoris
vaccine
DOI - identifier 10.2217/fvl.14.93
Copyright notice © 2014 Future Medicine Ltd
ISSN 1746-0794
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