Cell biological responses of osteoblasts on anodized nanotubular surface of a titanium-zirconium alloy

Sista, S, Nouri, A, Li, Y, Wen, C, Hodgson, P and Pande, G 2013, 'Cell biological responses of osteoblasts on anodized nanotubular surface of a titanium-zirconium alloy', Journal of Biomedical Materials Research - Part A, vol. 101, no. 12, pp. 3416-3430.


Document type: Journal Article
Collection: Journal Articles

Title Cell biological responses of osteoblasts on anodized nanotubular surface of a titanium-zirconium alloy
Author(s) Sista, S
Nouri, A
Li, Y
Wen, C
Hodgson, P
Pande, G
Year 2013
Journal name Journal of Biomedical Materials Research - Part A
Volume number 101
Issue number 12
Start page 3416
End page 3430
Total pages 15
Publisher John Wiley & Sons
Abstract Anodization of titanium and its alloys, under controlled conditions, generates a nanotubular architecture on the material surface. The biological consequences of such changes are poorly understood, and therefore, we have analyzed the cellular and molecular responses of osteoblasts that were plated on nanotubular anodized surface of a titanium-zirconium (TiZr) alloy. Upon comparing these results with those obtained on acid etched and polished surfaces of the same alloy, we observed a significant increase in adhesion and proliferation of cells on anodized surfaces as compared to acid etched or polished surface. The expression of genes related to cell adhesion was high only on anodized TiZr, but that of genes related to osteoblast differentiation and osteocalcin protein and extracellular matrix secretion were higher on both anodized and acid etched surfaces. Examination of surface morphology, topography, roughness, surface area and wettability using scanning electron microscopy, atomic force microscopy, and contact angle goniometry, showed that higher surface area, hydrophilicity, and nanoscale roughness of nanotubular TiZr surfaces, which were generated specifically by the anodization process, could strongly enhance the adhesion and proliferation of osteoblasts. We propose that biological properties of known bioactive titanium alloys can be further enhanced by generating nanotubular surfaces using anodization
Subject Biomaterials
Nanobiotechnology
Metals and Alloy Materials
Keyword(s) Anodization
Titanium alloy
Osteoblast
Gene expression
Nanotubes
DOI - identifier 10.1002/jbm.a.34638
Copyright notice © 2013 Wiley Periodicals, Inc.
ISSN 1549-3296
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