Application of a pathogenicity marker found in Escherichia coli for the assessment of irrigation water quality

Keeling, S, Moutafis, G, Hayman, B and Coloe, P 2007, 'Application of a pathogenicity marker found in Escherichia coli for the assessment of irrigation water quality', Water Environment Research, vol. 79, no. 5, pp. 561-566.


Document type: Journal Article
Collection: Journal Articles

Title Application of a pathogenicity marker found in Escherichia coli for the assessment of irrigation water quality
Author(s) Keeling, S
Moutafis, G
Hayman, B
Coloe, P
Year 2007
Journal name Water Environment Research
Volume number 79
Issue number 5
Start page 561
End page 566
Total pages 5
Publisher Water Environment Federation
Abstract A polymerase chain reaction (PCR)-based method was developed to differentiate between pathogenic and nonpathogenic Escherichia coli (E. coli). A pathogenicity marker, linked to the deletion of the ygfB gene, was identified in 80% of the clinical E. coli isolates tested. This marker, combined with the malic acid dehydrogenase gene, formed the duplex PCR that was subsequently used to screen E. coli isolates recovered from two secondary wastewater treatment plants (STPs) and a river site. All waters samples are used to irrigate dairy farm pasture in the West Gippsland region of Victoria, Australia. Results from three consecutive months of sampling (December 2001 and January and February 2002) indicated that Longwarry STP showed 8, 8, and 0% pathogenic E. coli; Pakenham STP showed 0, 12.5, and 33%; and the Bunyip river site showed 20, 12, and 25% respectively.
Subject Agricultural Hydrology (Drainage, Flooding, Irrigation, Quality, etc.)
Copyright notice © Water Environment Federation 2007
ISSN 1061-4303
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