Antibiotic resistance in food-borne bacterial contaminants in Vietnam

Van, T, Moutafis, G, Thuoc, T and Coloe, P 2007, 'Antibiotic resistance in food-borne bacterial contaminants in Vietnam', Applied And Environmental Microbiology, vol. 73, pp. 7906-7911.

Document type: Journal Article
Collection: Journal Articles

Title Antibiotic resistance in food-borne bacterial contaminants in Vietnam
Author(s) Van, T
Moutafis, G
Thuoc, T
Coloe, P
Year 2007
Journal name Applied And Environmental Microbiology
Volume number 73
Start page 7906
End page 7911
Total pages 5
Publisher American Society for Microbiology
Abstract This study was conducted to examine the rate of contamination and the molecular characteristics of enteric bacteria isolated from a selection of food sources in Vietnam. One hundred eighty raw food samples were tested; 60.8% of meat samples and 18.0% of shellfish samples were contaminated with Salmonella spp., and more than 90% of all food sources contained Escherichia coli. The isolates were screened for antibiotic resistance against 15 antibiotics, and 50.5% of Salmonella isolates and 83.8% of E. coli isolates were resistant to at least one antibiotic. Isolates were examined for the presence of mobile genetic elements conferring antibiotic resistance. Fifty-seven percent of E. coli and 13% of Salmonella isolates were found to contain integrons, and some isolates contained two integrons. Sequencing results revealed that the integrons harbored various gene cassettes, including aadA1, aadA2, and aad45 (resistance to streptomycin and spectinomycin), aacA4 (resistance to aminoglycosides), the dihydrofolate reductase gene cassettes dhfrXII, dftA1, and dhfrA17 (trimethoprim resistance), the beta-lactamase gene bla(PSE1) (ampicillin resistance), and catB3 (chloramphenicol resistance). Plasmids were also detected in all 23 antibiotic-resistant Salmonella isolates and in 33 E. coli isolates. Thirty-five percent of the Salmonella isolates and 76% of the E. coli isolates contained plasmids of more than 95 kb, and some of the isolates contained two large plasmids. Conjugation experiments showed the successful transfer of all or part of the antibiotic resistance phenotypes among the Salmonella and E. coli food isolates. Our results show that enteric bacteria in raw food samples from Vietnam contain a pool of mobile genetic elements and that the transfer of antibiotic resistance can readily occur between similar bacteria.
Subject Infectious Agents
DOI - identifier 10.1128/aem.00973-07
Copyright notice Copyright © 2007, American Society for Microbiology.
ISSN 0099-2240
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