An SECM study on the influence of cationic, membrane-active peptides on a gold-supported self-assembled monolayer

Abdelhamid, M, Piantavigna, S, Bond, A, Graham, B, Spiccia, L, Martin, L and O'Mullane, A 2015, 'An SECM study on the influence of cationic, membrane-active peptides on a gold-supported self-assembled monolayer', Electrochemistry Communications, vol. 51, pp. 11-14.


Document type: Journal Article
Collection: Journal Articles

Title An SECM study on the influence of cationic, membrane-active peptides on a gold-supported self-assembled monolayer
Author(s) Abdelhamid, M
Piantavigna, S
Bond, A
Graham, B
Spiccia, L
Martin, L
O'Mullane, A
Year 2015
Journal name Electrochemistry Communications
Volume number 51
Start page 11
End page 14
Total pages 4
Publisher Elsevier Inc.
Abstract The influence of the membrane active peptides, Tat44-57 (activator in HIV-1) and melittin (active content of bee venom), on self-assembled monolayers of 6-mercaptohexanoic acid (MHA) on gold electrodes has been studied with scanning electrochemical microscopy (SECM). It was found that MHA, when deprotonated at physiological pH, significantly affected the relative rates of electron transfer between the [Fe(CN)6]4- solution based mediator and the underlying gold electrode, predominantly by the electrostatic interaction between the mediator and MHA. Upon the introduction of Tat44-57 ormelittin to the electrolyte, the relative rate of electron transfer through the MHA layer could be increased or decreased depending on the mediator used. However, in all cases it was found that these peptides have the ability to be incorporated into synthetic SAMs, which has implications for future electrochemical studies carried out using cell mimicking membranes immobilised on such layers.
Subject Electrochemistry
Proteins and Peptides
Keyword(s) Electron transfer
Peptides
SECM
Self-assembled monolayers
DOI - identifier 10.1016/j.elecom.2014.11.018
Copyright notice © 2014 Elsevier B.V.
ISSN 1388-2481
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