A hydrodynamic microchip for formation of continuous cell chains

Khoshmanesh, K, Zhang, W, Tang, S, Nasabi, M, Soffe, R, Tovar-Lopez, F, Rajadas, J and Mitchell, A 2014, 'A hydrodynamic microchip for formation of continuous cell chains', Applied Physics Letters, vol. 104, no. 20, 203701, pp. 1-5.


Document type: Journal Article
Collection: Journal Articles

Title A hydrodynamic microchip for formation of continuous cell chains
Author(s) Khoshmanesh, K
Zhang, W
Tang, S
Nasabi, M
Soffe, R
Tovar-Lopez, F
Rajadas, J
Mitchell, A
Year 2014
Journal name Applied Physics Letters
Volume number 104
Issue number 20
Article Number 203701
Start page 1
End page 5
Total pages 5
Publisher American Institute of Physics
Abstract Here, we demonstrate the unique features of a hydrodynamic based microchip for creating continuous chains of model yeast cells. The system consists of a disk shaped microfluidic structure, containing narrow orifices that connect the main channel to an array of spoke channels. Negative pressure provided by a syringe pump draws fluid from the main channel through the narrow orifices. After cleaning process, a thin layer of water is left between the glass substrate and the polydimethylsiloxane microchip, enabling leakage beneath the channel walls. A mechanical clamp is used to adjust the operation of the microchip. Relaxing the clamp allows leakage of liquid beneath the walls in a controllable fashion, leading to formation of a long cell chain evenly distributed along the channel wall. The unique features of the microchip are demonstrated by creating long chains of yeast cells and model 15μm polystyrene particles along the side wall and analysing the hydrogen peroxide induced death of patterned cells.
Subject Physical Sciences not elsewhere classified
Engineering not elsewhere classified
Keyword(s) Chains
Channel flow
Cytology
Hydrodynamics
Microprocessor chips
Orifices
Silicones
Substrates
Yeast Cleaning process
Glass substrates
Microfluidic structures
Negative pressures
Polydimethylsiloxane microchips
Polystyrene particle
Syringe pumps
Unique features
DOI - identifier 10.1063/1.4879457
Copyright notice © 2014 AIP Publishing LLC
ISSN 0003-6951
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