Brazilian green propolis and its constituent, Artepillin C inhibits allogeneic activated human CD4 T cells expansion and activation

Cheung, K, Sze, M, Chan, W, Deng, R, Tu, W and Chan, G 2011, 'Brazilian green propolis and its constituent, Artepillin C inhibits allogeneic activated human CD4 T cells expansion and activation', Journal of Ethnopharmacology, vol. 138, no. 2, pp. 463-471.


Document type: Journal Article
Collection: Journal Articles

Title Brazilian green propolis and its constituent, Artepillin C inhibits allogeneic activated human CD4 T cells expansion and activation
Author(s) Cheung, K
Sze, M
Chan, W
Deng, R
Tu, W
Chan, G
Year 2011
Journal name Journal of Ethnopharmacology
Volume number 138
Issue number 2
Start page 463
End page 471
Total pages 9
Publisher Elsevier Ireland Ltd.
Abstract Ethnopharmacological relevance: Propolis has long been used as a popular folk medicine by various ethnic groups due to its wide spectrum of alleged biological and pharmaceutical properties including anti-microbial, anti-cancer and anti-inflammatory functions. All these can be linked to the modulation of immune function. Therefore, it will be relevant for us to find out whether there is any novel compound that can account for such action and the mechanism involved. Aim of the study: We investigated the immune modulating effect of Brazilian green propolis (PBrazil) and its constituent Artepillin C (Art-C) by using mixed leukocytes reaction. Materials and methods: The cytotoxic effect of Art-C on non-tumorigenic human liver cell line miHA and non-tumorigenic human kidney cell line HK-2 as well as human peripheral blood mononuclear cells (PBMCs) were measured by XTT cell proliferation assay. The effect of PBrazil and Art-C on T cell proliferation and activation were determined by using carboxyfluorescein succinimidyl ester (CFSE) and by CD25 expression, respectively. Cytokines including tumor necrosis factor-α (TNF-α), interferon-gamma (IFN-γ), interleukins such as IL-2, IL-17 were measured by intracellular cytokine staining and IL-10 was measured by ELISA. The effect of PBrazil and Art-C on regulatory T cells (Treg) induction was determined by the Foxp3 expression. The apoptotic effect of these compounds on CFSE labeled alloreactive T cells was measured by using Annexin V.
Subject Tumour Immunology
Keyword(s) Artepillin C
Brazilian green propolis
Mixed leukocyte reaction
Propolis
T cell
T cell activation
DOI - identifier 10.1016/j.jep.2011.09.031
Copyright notice © 2011 Elsevier Ireland Ltd. All rights reserved.
ISSN 0378-8741
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