Anti-amyloidogenic properties of some phenolic compounds

Porzoor, A, Alford, B, Hugel, H, Grando, D, Caine, J and Macreadie, I 2015, 'Anti-amyloidogenic properties of some phenolic compounds', Biomolecules, vol. 5, no. 2, pp. 505-527.


Document type: Journal Article
Collection: Journal Articles

Title Anti-amyloidogenic properties of some phenolic compounds
Author(s) Porzoor, A
Alford, B
Hugel, H
Grando, D
Caine, J
Macreadie, I
Year 2015
Journal name Biomolecules
Volume number 5
Issue number 2
Start page 505
End page 527
Total pages 23
Publisher MDPI AG
Abstract A family of 21 polyphenolic compounds consisting of those found naturally in danshen and their analogues were synthesized and subsequently screened for their anti-amyloidogenic activity against the amyloid beta peptide (A beta(42)) of Alzheimer's disease. After 24 h incubation with A beta(42), five compounds reduced thioflavin T (ThT) fluorescence, indicative of their anti-amyloidogenic propensity (p < 0.001). TEM and immunoblotting analysis also showed that selected compounds were capable of hindering fibril formation even after prolonged incubations. These compounds were also capable of rescuing the yeast cells from toxic changes induced by the chemically synthesized A beta(42). In a second assay, a Saccharomyces cerevisiae AHP1 deletant strain transformed with GFP fused to A beta(42) was treated with these compounds and analyzed by flow cytometry. There was a significant reduction in the green fluorescence intensity associated with 14 compounds. We interpret this result to mean that the compounds had an anti-amyloid-aggregation propensity in the yeast and GFP-A beta(42) was removed by proteolysis. The position and not the number of hydroxyl groups on the aromatic ring was found to be the most important determinant for the anti-amyloidogenic properties.
Subject Organic Chemistry not elsewhere classified
Keyword(s) Alzheimer's disease
Saccharomyces cerevisiae
polyphenols
AHP1
anti-amyloidogenic
danshen
DOI - identifier 10.3390/biom5020505
Copyright notice © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license. (https://creativecommons.org/licenses/by/4.0/)
ISSN 2218-273X
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