Breaking the diffraction-limited resolution barrier in fiber-optical two-photon fluorescence endoscopy by an azimuthally-polarized beam

Gu, M, Kang, H and Li, X 2014, 'Breaking the diffraction-limited resolution barrier in fiber-optical two-photon fluorescence endoscopy by an azimuthally-polarized beam', Scientific Reports, vol. 4, 3627, pp. 1-6.


Document type: Journal Article
Collection: Journal Articles

Title Breaking the diffraction-limited resolution barrier in fiber-optical two-photon fluorescence endoscopy by an azimuthally-polarized beam
Author(s) Gu, M
Kang, H
Li, X
Year 2014
Journal name Scientific Reports
Volume number 4
Article Number 3627
Start page 1
End page 6
Total pages 6
Publisher Nature Publishing Group
Abstract Although fiber-optical two-photon endoscopy has been recognized as a potential high-resolution diagnostic and therapeutic procedure in vivo, its resolution is limited by the optical diffraction nature to a few micrometers due to the low numerical aperture of an endoscopic objective. On the other hand, stimulated emission depletion (STED) achieved by a circularly-polarized vortex beam has been used to break the diffraction-limited resolution barrier in a bulky microscope. It has been a challenge to apply the STED principle to a fiber-optical two-photon endoscope as a circular polarization state cannot be maintained due to the birefringence of a fiber. Here, we demonstrate the first fiber-optical STED two-photon endoscope using an azimuthally-polarized beam directly generated from a double-clad fiber. As such, the diffraction-limited resolution barrier of fiber-optical two-photon endoscopy can be broken by a factor of three. Our new accomplishment has paved a robust way for high-resolution in vivo biomedical studies.
Subject Biomedical Engineering not elsewhere classified
Keyword(s) article
endoscopy
equipment
fiber optics
fluorescence
methodology
DOI - identifier 10.1038/srep03627
Copyright notice This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
ISSN 2045-2322
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