Antibiotic regimen based on population analysis of residing persister cells eradicates Staphylococcus epidermidis biofilms

Yang, S, Hay, I, Cameron, D, Speir, M, Cui, B, Su, F, Peleg, A, Lithgow, T, Deighton, M and Qu, Y 2015, 'Antibiotic regimen based on population analysis of residing persister cells eradicates Staphylococcus epidermidis biofilms', Scientific Reports, vol. 5, 18578, pp. 1-11.


Document type: Journal Article
Collection: Journal Articles

Title Antibiotic regimen based on population analysis of residing persister cells eradicates Staphylococcus epidermidis biofilms
Author(s) Yang, S
Hay, I
Cameron, D
Speir, M
Cui, B
Su, F
Peleg, A
Lithgow, T
Deighton, M
Qu, Y
Year 2015
Journal name Scientific Reports
Volume number 5
Article Number 18578
Start page 1
End page 11
Total pages 11
Publisher Nature Publishing Group
Abstract Biofilm formation is a major pathogenicity strategy of Staphylococcus epidermidis causing various medical-device infections. Persister cells have been implicated in treatment failure of such infections. We sought to profile bacterial subpopulations residing in S. epidermidis biofilms, and to establish persister-targeting treatment strategies to eradicate biofilms. Population analysis was performed by challenging single biofilm cells with antibiotics at increasing concentrations ranging from planktonic minimum bactericidal concentrations (MBCs) to biofilm MBCs (MBCbiofilm). Two populations of "persister cells" were observed: bacteria that survived antibiotics at MBCbiofilm for 24/48 hours were referred to as dormant cells; those selected with antibiotics at 8 X MICs for 3 hours (excluding dormant cells) were defined as tolerant-but-killable (TBK) cells. Antibiotic regimens targeting dormant cells were tested in vitro for their efficacies in eradicating persister cells and intact biofilms. This study confirmed that there are at least three subpopulations within a S. epidermidis biofilm: normal cells, dormant cells, and TBK cells. Biofilms comprise more TBK cells and dormant cells than their log-planktonic counterparts. Using antibiotic regimens targeting dormant cells, i.e. effective antibiotics at MBCbiofilm for an extended period, might eradicate S. epidermidis biofilms. Potential uses for this strategy are in antibiotic lock techniques and inhaled aerosolized antibiotics.
Subject Physical Sciences not elsewhere classified
DOI - identifier 10.1038/srep18578
Copyright notice This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
ISSN 2045-2322
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