Apocynin and ebselen reduce influenza A virus-induced lung inflammation in cigarette smoke-exposed mice

Oostwoud, L, Gunasinghe, P, Seow, H, Ye, J, Selemidis, S, Bozinovski, S and Vlahos, R 2016, 'Apocynin and ebselen reduce influenza A virus-induced lung inflammation in cigarette smoke-exposed mice', Scientific Reports, vol. 6, 20983, pp. 1-16.


Document type: Journal Article
Collection: Journal Articles

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Title Apocynin and ebselen reduce influenza A virus-induced lung inflammation in cigarette smoke-exposed mice
Author(s) Oostwoud, L
Gunasinghe, P
Seow, H
Ye, J
Selemidis, S
Bozinovski, S
Vlahos, R
Year 2016
Journal name Scientific Reports
Volume number 6
Article Number 20983
Start page 1
End page 16
Total pages 16
Publisher Nature Publishing Group
Abstract Influenza A virus (IAV) infections are a common cause of acute exacerbations of chronic obstructive pulmonary disease (AECOPD). Oxidative stress is increased in COPD, IAV-induced lung inflammation and AECOPD. Therefore, we investigated whether targeting oxidative stress with the Nox2 oxidase inhibitors and ROS scavengers, apocynin and ebselen could ameliorate lung inflammation in a mouse model of AECOPD. Male BALB/c mice were exposed to cigarette smoke (CS) generated from 9 cigarettes per day for 4 days. On day 5, mice were infected with 1 × 104.5 PFUs of the IAV Mem71 (H3N1). BALF inflammation, viral titers, superoxide production and whole lung cytokine, chemokine and protease mRNA expression were assessed 3 and 7 days post infection. IAV infection resulted in a greater increase in BALF inflammation in mice that had been exposed to CS compared to non-smoking mice. This increase in BALF inflammation in CS-exposed mice caused by IAV infection was associated with elevated gene expression of pro-inflammatory cytokines, chemokines and proteases, compared to CS alone mice. Apocynin and ebselen significantly reduced the exacerbated BALF inflammation and pro-inflammatory cytokine, chemokine and protease expression caused by IAV infection in CS mice. Targeting oxidative stress using apocynin and ebselen reduces IAV-induced lung inflammation in CS-exposed mice and may be therapeutically exploited to alleviate AECOPD.
Subject Respiratory Diseases
DOI - identifier 10.1038/srep20983
Copyright notice Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
ISSN 2045-2322
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