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Apocynin and ebselen reduce influenza A virus-induced lung inflammation in cigarette smoke-exposed mice Oostwoud, L, Gunasinghe, P, Seow, H, Ye, J, Selemidis, S, Bozinovski, S and Vlahos, R 2016, 'Apocynin and ebselen reduce influenza A virus-induced lung inflammation in cigarette smoke-exposed mice', Scientific Reports, vol. 6, 20983, pp. 1-16. Document type: Journal Article Collection: Journal Articles Attached Files Name Description MIMEType Size n2006059582.pdf Published Version application/pdf 1.65MB Title Apocynin and ebselen reduce influenza A virus-induced lung inflammation in cigarette smoke-exposed mice Author(s) Oostwoud, L Gunasinghe, P Seow, H Ye, J Selemidis, S Bozinovski, S Vlahos, R Year 2016 Journal name Scientific Reports Volume number 6 Article Number 20983 Start page 1 End page 16 Total pages 16 Publisher Nature Publishing Group Abstract Influenza A virus (IAV) infections are a common cause of acute exacerbations of chronic obstructive pulmonary disease (AECOPD). Oxidative stress is increased in COPD, IAV-induced lung inflammation and AECOPD. Therefore, we investigated whether targeting oxidative stress with the Nox2 oxidase inhibitors and ROS scavengers, apocynin and ebselen could ameliorate lung inflammation in a mouse model of AECOPD. Male BALB/c mice were exposed to cigarette smoke (CS) generated from 9 cigarettes per day for 4 days. On day 5, mice were infected with 1 × 104.5 PFUs of the IAV Mem71 (H3N1). BALF inflammation, viral titers, superoxide production and whole lung cytokine, chemokine and protease mRNA expression were assessed 3 and 7 days post infection. IAV infection resulted in a greater increase in BALF inflammation in mice that had been exposed to CS compared to non-smoking mice. This increase in BALF inflammation in CS-exposed mice caused by IAV infection was associated with elevated gene expression of pro-inflammatory cytokines, chemokines and proteases, compared to CS alone mice. Apocynin and ebselen significantly reduced the exacerbated BALF inflammation and pro-inflammatory cytokine, chemokine and protease expression caused by IAV infection in CS mice. Targeting oxidative stress using apocynin and ebselen reduces IAV-induced lung inflammation in CS-exposed mice and may be therapeutically exploited to alleviate AECOPD. Subject Respiratory Diseases DOI - identifier 10.1038/srep20983 Copyright notice This work is licensed under a Creative Commons Attribution 4.0 International License. ISSN 2045-2322 Related Links Link Description http://dx.doi.org/10.1038/srep20983 Link to Published Version   Versions Version Filter Type Wed, 23 Mar 2016, 08:53:56 EST Tue, 26 Jul 2016, 09:02:50 EST Mon, 10 Jul 2017, 12:32:39 EST Mon, 10 Jul 2017, 13:18:35 EST Tue, 05 Dec 2017, 09:58:57 EST Tue, 05 Dec 2017, 09:59:42 EST Filtered Full Citation counts:   Cited 26 times in Thomson Reuters Web of Science Article | Citations Cited 5 times in Scopus Article | Citations Altmetric details: Access Statistics: 167 Abstract Views, 56 File Downloads  -  Detailed Statistics Created: Wed, 23 Mar 2016, 08:48:00 EST by Catalyst Administrator