Assessment of the potential of a high frequency acoustomicrofluidic nebulisation platform for inhaled stem cell therapy

Alhasan, L, Qi, A, Rezk, A, Yeo, L and Chan, P 2016, 'Assessment of the potential of a high frequency acoustomicrofluidic nebulisation platform for inhaled stem cell therapy', Integrative Biology (United Kingdom), vol. 8, no. 1, pp. 12-20.


Document type: Journal Article
Collection: Journal Articles

Title Assessment of the potential of a high frequency acoustomicrofluidic nebulisation platform for inhaled stem cell therapy
Author(s) Alhasan, L
Qi, A
Rezk, A
Yeo, L
Chan, P
Year 2016
Journal name Integrative Biology (United Kingdom)
Volume number 8
Issue number 1
Start page 12
End page 20
Total pages 9
Publisher Royal Society of Chemistry
Abstract Despite the promise of stem cell therapy for lung therapeutics and repair, there are few viable means for directly delivering stem cells to locally target the respiratory airways via inhalation. This is not surprising given the significant challenges in aerosolising stem cells, particularly given their susceptibility to damage under the large stresses involved in the nebulisation process. Here, we present promising results using a microfluidic acoustic nebulisation platform that is not only low cost and portable, but also its high MHz order frequencies are effective for preserving the structural and functional integrity of mesenchymal stem cells (MSCs) during the nebulisation process. This is verified through an assessment of the viability, structure, metabolic activity, proliferation ability and genetic makeup of the nebulised MSCs using a variety of assays, including cell viability staining, flow cytometry, reverse transcription and quantitative polymerase chain reaction, and immunophenotyping, thus demonstrating the platform as a promising method for efficient pulmonary stem cell delivery.
Subject Medicinal and Biomolecular Chemistry not elsewhere classified
DOI - identifier 10.1039/c5ib00206k
Copyright notice © The Royal Society of Chemistry 2016
ISSN 1757-9694
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