A quantitative PCR approach for quantification of functional genes involved in the degradation of polycyclic aromatic hydrocarbons in contaminated soils

Shahsavari, E, Aburto Medina, A, Taha Elfouly, M and Ball, A 2016, 'A quantitative PCR approach for quantification of functional genes involved in the degradation of polycyclic aromatic hydrocarbons in contaminated soils', MethodsX, vol. 3, pp. 205-211.


Document type: Journal Article
Collection: Journal Articles

Title A quantitative PCR approach for quantification of functional genes involved in the degradation of polycyclic aromatic hydrocarbons in contaminated soils
Author(s) Shahsavari, E
Aburto Medina, A
Taha Elfouly, M
Ball, A
Year 2016
Journal name MethodsX
Volume number 3
Start page 205
End page 211
Total pages 7
Publisher Elsevier
Abstract Polycyclic aromatic hydrocarbons (PAHs) are major pollutants globally and due to their carcinogenic and mutagenic properties their clean-up is paramount. Bioremediation or using PAH degrading microorganisms (mainly bacteria) to degrade the pollutants represents cheap, effective methods. These PAH degraders harbor functional genes which help microorganisms use PAHs as source of food and energy. Most probable number (MPN) and plate counting methods are widely used for counting PAHs degraders; however, as culture based methods only count a small fraction (<1%) of microorganisms capable of carrying out PAH degradation, the use of culture-independent methodologies is desirable.This protocol presents a robust, rapid and sensitive qPCR method for the quantification of the functional genes involved in the degradation of PAHs in soil samples.This protocol enables us to screen a vast number of PAH contaminated soil samples in few hours.This protocol provides valuable information about the natural attenuation potential of contaminated soil and can be used to monitor the bioremediation process.
Subject Bioremediation
Keyword(s) Bioremediation
Functional genes
Hydrocarbons
PAHs
PCR
qPCR
DOI - identifier 10.1016/j.mex.2016.02.005
Copyright notice © 2016 The Authors
ISSN 2215-0161
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