18 GHz electromagnetic field induces permeability of Gram-positive cocci

Nguyen, T, Shamis, Y, Croft, R, Wood, A, McIntosh, R, Crawford, R and Ivanova, E 2015, '18 GHz electromagnetic field induces permeability of Gram-positive cocci', Scientific Reports, vol. 5, 10980, pp. 1-12.

Document type: Journal Article
Collection: Journal Articles

Title 18 GHz electromagnetic field induces permeability of Gram-positive cocci
Author(s) Nguyen, T
Shamis, Y
Croft, R
Wood, A
McIntosh, R
Crawford, R
Ivanova, E
Year 2015
Journal name Scientific Reports
Volume number 5
Article Number 10980
Start page 1
End page 12
Total pages 12
Publisher Nature Publishing Group
Abstract The effect of electromagnetic field (EMF) exposures at the microwave (MW) frequency of 18 GHz, on four cocci, Planococcus maritimus KMM 3738, Staphylococcus aureus CIP 65.8T, S. aureus ATCC 25923 and S. epidermidis ATCC 14990T, was investigated. We demonstrate that exposing the bacteria to an EMF induced permeability in the bacterial membranes of all strains studied, as confirmed directly by transmission electron microscopy (TEM), and indirectly via the propidium iodide assay and the uptake of silica nanospheres. The cells remained permeable for at least nine minutes after EMF exposure. It was shown that all strains internalized 23.5 nm nanospheres, whereas the internalization of the 46.3 nm nanospheres differed amongst the bacterial strains (S. epidermidis ATCC 14990T~ 0%; Staphylococcus aureus CIP 65.8T S. aureus ATCC 25923, ~40%; Planococcus maritimus KMM 3738, ~80%). Cell viability experiments indicated that up to 84% of the cells exposed to the EMF remained viable. The morphology of the bacterial cells was not altered, as inferred from the scanning electron micrographs, however traces of leaked cytosolic fluids from the EMF exposed cells could be detected. EMF-induced permeabilization may represent an innovative, alternative cell permeability technique for applications in biomedical engineering, cell drug delivery and gene therapy.
Subject Physical Chemistry not elsewhere classified
Microbiology not elsewhere classified
Materials Engineering not elsewhere classified
Keyword(s) Molecular-Dynamics Simulations
Optical Transfection
Biological Tissues
DOI - identifier 10.1038/srep10980
Copyright notice © 2015 Creative Commons Attribution
ISSN 2045-2322
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Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
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