A novel rat model of chronic heart failure following myocardial infarction

Fu, Y, Lin, Q, Li, X, Feih, H, Shan, Z, Huang, X, Liu, X, Yang, M, Lin, S and Zhou, S 2009, 'A novel rat model of chronic heart failure following myocardial infarction', Methods and Findings in Experimental and Clinical Pharmacology, vol. 31, no. 6, pp. 367-373.


Document type: Journal Article
Collection: Journal Articles

Title A novel rat model of chronic heart failure following myocardial infarction
Author(s) Fu, Y
Lin, Q
Li, X
Feih, H
Shan, Z
Huang, X
Liu, X
Yang, M
Lin, S
Zhou, S
Year 2009
Journal name Methods and Findings in Experimental and Clinical Pharmacology
Volume number 31
Issue number 6
Start page 367
End page 373
Total pages 7
Publisher Prous Science
Abstract In this report, we describe an improved method for the establishment of reproducible congestive heart failure (CHF) in a rat model. The area of myocardial infarction (MI) after ligation of the left anterior descending (LAD) coronary artery was quantified. Histological changes, heart function detected by echocardiography and isolated Langendorff perfusion, and selected biochemical factors were monitored after ligation of the LAD. Contrary to previous beliefs, thoracotomy in the second intercostal space provided a much better visualization of and easier access to the LAD and significantly reduced the mortality rate. Surface electrocardiogram (ECG) showed that the S-T interval was arched raised upward immediately after ligation. Typical morphological and functional changes of CHF were observed after LAD ligation. Cardiomyocytes in the infarcted zone were depleted and deranged. Biochemical analysis and enzyme-linked immunosorbent assay (ELISA) showed that superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and nitric oxide (NO) levels were significantly lowered in rats with MI than in the normal and sham groups, whereas serum malondialdehyde (MDA), MB isoenzyme of creatine kinase (CK-MB), cardiac troponin (cTnT) and C-reactive protein (CRP) levels were elevated. After MI, N-terminal pro-brain natriuretic peptide (NT-proBNP) was increased but insulin-like growth factor I (IGF-I) and vascular endothelial growth factor (VEGF) in culture supernatant were lower than in the normal and sham groups. We present an improved model for maximal reproducibility of experimental CHF in rats which allows the study of molecular and physiological variables in relation to CHF.
Subject Cardiology (incl. Cardiovascular Diseases)
Keyword(s) rat
chronic heart failure
myocardial infarct
isolated Langendorff heart perfusion
Copyright notice © 2009 Prous Science, S.A.U or Its licensors. All rights reserved,
ISSN 0379-0355
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