Brightness and photostability of emerging red and near-IR fluorescent nanomaterials for bioimaging

Reineck, P, Francis, A, Orth, A, Lau, D, Nixon-Luke, R, Rastogi, I, Razali, W, Cordina, N, Parker,, L, Sreenivasan, V, Brown, L and Gibson, B 2016, 'Brightness and photostability of emerging red and near-IR fluorescent nanomaterials for bioimaging', Advanced Optical Materials, vol. 4, no. 10, pp. 1549-1557.


Document type: Journal Article
Collection: Journal Articles

Title Brightness and photostability of emerging red and near-IR fluorescent nanomaterials for bioimaging
Author(s) Reineck, P
Francis, A
Orth, A
Lau, D
Nixon-Luke, R
Rastogi, I
Razali, W
Cordina, N
Parker,, L
Sreenivasan, V
Brown, L
Gibson, B
Year 2016
Journal name Advanced Optical Materials
Volume number 4
Issue number 10
Start page 1549
End page 1557
Total pages 9
Publisher Wiley
Abstract Many novel fluorescent nanomaterials exhibit radically different optical properties compared to organic fluorophores that are still the most extensively used class of fluorophores in biology today. Assessing the practical impact of these optical differences for bioimaging experiments is challenging due to a lack of published quantitative benchmarking data. This study therefore directly and quantitatively compares the brightness and photostability of representatives from seven classes of fluorescent materials in spectroscopy and fluorescence microscopy experiments for the first time. These material classes are: organic dyes, semiconductor quantum dots, fluorescent beads, carbon dots, gold nanoclusters, nanodiamonds, and nanorubies. The relative brightness of each material is determined and the minimum material concentrations required to generate sufficient contrast in a fluorescence microscopy image are assessed. The influence of optical filters used for imaging is also discussed and suitable filter combinations are identified. The photostability of all materials is determined under typical imaging conditions and the number of images that can be acquired is inferred. The results are expected to facilitate the transition of novel fluorescent materials from physics and chemistry into biology laboratories.
Subject Condensed Matter Imaging
Keyword(s) bioimaging
brightness
fluorescence
nanomaterials
photostability
DOI - identifier 10.1002/adom.201600212
Copyright notice © 2016 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim
ISSN 2195-1071
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