13C metabolomics reveals widespread change in carbon fate during coral bleaching

Hillyer, K, Dias, D, Lutz, A, Roessner, U and Davy, S 2018, '13C metabolomics reveals widespread change in carbon fate during coral bleaching', Metabolomics, vol. 14, no. 12, pp. 1-9.

Document type: Journal Article
Collection: Journal Articles

Title 13C metabolomics reveals widespread change in carbon fate during coral bleaching
Author(s) Hillyer, K
Dias, D
Lutz, A
Roessner, U
Davy, S
Year 2018
Journal name Metabolomics
Volume number 14
Issue number 12
Start page 1
End page 9
Total pages 9
Publisher Springer New York
Abstract Introduction: Rising seawater temperatures are threatening the persistence of coral reefs; where above critical thresholds, thermal stress results in a breakdown of the coral-dinoflagellate symbiosis and the loss of algal symbionts (coral bleaching). As symbiont-derived organic products typically form a major portion of host energy budgets, this has major implications for the fitness and persistence of symbiotic corals. Objectives: We aimed to determine change in autotrophic carbon fate within individual compounds and downstream metabolic pathways in a coral symbiosis exposed to varying degrees of thermal stress and bleaching. Methods: We applied gas chromatography-mass spectrometry coupled to a stable isotope tracer (13C), to track change in autotrophic carbon fate, in symbiont and host individually, following exposure to elevated water temperature. Results: Thermal stress resulted in partner-specific changes in carbon fate, which progressed with heat stress duration. We detected modifications to carbohydrate and fatty acid metabolism, lipogenesis, and homeostatic responses to thermal, oxidative and osmotic stress. Despite pronounced photodamage, remaining in hospite symbionts continued to produce organic products de novo and translocate to the coral host. However as bleaching progressed, we observed minimal 13C enrichment of symbiont long-chain fatty acids, also reflected in 13C enrichment of host fatty acid pools. Conclusion: These data have major implications for our understanding of coral symbiosis function during bleaching. Our findings suggest that during early stage bleaching, remaining symbionts continue to effectively translocate a variety of organic products to the host, however under prolonged thermal stress there is likely a reduction in the quality of these products.
Subject Medical Biochemistry and Metabolomics not elsewhere classified
Analytical Biochemistry
Ecological Impacts of Climate Change
Keyword(s) Cnidarian Symbiodinium
Stable isotope tracer
DOI - identifier 10.1007/s11306-017-1306-8
Copyright notice © Springer Science and Business Media 2017
ISSN 1573-3890
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
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