Lab-on-a-Chip imaging micro-echocardiography (i mu EC) for rapid assessment of cardiovascular activity in zebrafish larvae

Mohd Azmi, N, Kaslin, J and Wlodkowic, D 2018, 'Lab-on-a-Chip imaging micro-echocardiography (i mu EC) for rapid assessment of cardiovascular activity in zebrafish larvae', Sensors and Actuators, B: Chemical, vol. 256, pp. 1131-1141.


Document type: Journal Article
Collection: Journal Articles

Title Lab-on-a-Chip imaging micro-echocardiography (i mu EC) for rapid assessment of cardiovascular activity in zebrafish larvae
Author(s) Mohd Azmi, N
Kaslin, J
Wlodkowic, D
Year 2018
Journal name Sensors and Actuators, B: Chemical
Volume number 256
Start page 1131
End page 1141
Total pages 11
Publisher Elsevier
Abstract Analysis of physiological parameters using small model organisms is an attractive approach in pre-clinical drug discovery when studying effects of new drugs or existing drug combinations. Larval zebrafish (Danio rerio) is a small vertebrate model organism that recently emerged as an innovative experimental system for non-invasive and real-time analysis of physiological parameters such as cardiovascular activity. In this work, we demonstrate a new chip-enabled approach to perform non-invasive imaging micro-echocardiography (iμEC) on immobilized larval stages of zebrafish. The iμEC is a new assay for quantification of the temporal cardiac patterns based on analysis of video stream from a high-resolution camera. The chip-based system utilized hydrodynamic trapping principles to actively capture and immobilize individual larvae using a low-pressure suction for real-time iμEC analysis. The system coupled together with an integrated interface provided a new capability to rapidly assess pharmacologically-induced perturbation in cardiac function by real-time and non-invasive monitoring of zebrafish larvae. We demonstrated the application of the technology for proof-of-concept assessment of model cardio-active compounds delivered using continuous micro-perfusion. The optical transparency of larval stages and micro-hydrodynamic immobilization allowed for visualization of cardiac activity after drug treatment in real-time. Results obtained using micro-perfusion also enabl ed studies on rapid drug withdrawal that are difficult to achieve using existing protocols such as imaging in microtiter plates. This work demonstrates iμEC as a new technique for cardiological experiments utilizing zebrafish models.
Subject Animal Physiology - Systems
Microelectromechanical Systems (MEMS)
Keyword(s) Cardiology
Heart
Lab-on-a-Chip
Microfluidics
Toxicity
Zebrafish
DOI - identifier 10.1016/j.snb.2017.10.050
Copyright notice © 2017 Elsevier B.V.
ISSN 0925-4005
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