An organic fluorophore-nanodiamond hybrid sensor for photostable imaging and orthogonal, on-demand biosensing

Purdey, M, Capon, P, Pullen, B, Reineck, P, Schwarz, N, Psaltis, P, Nicholls, S, Gibson, B and Abell, A 2017, 'An organic fluorophore-nanodiamond hybrid sensor for photostable imaging and orthogonal, on-demand biosensing', Scientific Reports, vol. 7, no. 1, pp. 1-8.


Document type: Journal Article
Collection: Journal Articles

Title An organic fluorophore-nanodiamond hybrid sensor for photostable imaging and orthogonal, on-demand biosensing
Author(s) Purdey, M
Capon, P
Pullen, B
Reineck, P
Schwarz, N
Psaltis, P
Nicholls, S
Gibson, B
Abell, A
Year 2017
Journal name Scientific Reports
Volume number 7
Issue number 1
Start page 1
End page 8
Total pages 8
Publisher Nature
Abstract Organic fluorescent probes are widely used to detect key biomolecules; however, they often lack the photostability required for extended intracellular imaging. Here we report a new hybrid nanomaterial (peroxynanosensor, PNS), consisting of an organic fluorescent probe bound to a nanodiamond, that overcomes this limitation to allow concurrent and extended cell-based imaging of the nanodiamond and ratiometric detection of hydrogen peroxide. Far-red fluorescence of the nanodiamond offers continuous monitoring without photobleaching, while the green fluorescence of the organic fluorescent probe attached to the nanodiamond surface detects hydrogen peroxide on demand. PNS detects basal production of hydrogen peroxide within M1 polarised macrophages and does not affect macrophage growth during prolonged co-incubation. This nanosensor can be used for extended bio-imaging not previously possible with an organic fluorescent probe, and is spectrally compatible with both Hoechst 33342 and MitoTracker Orange stains for hyperspectral imaging.
Subject Physical Chemistry of Materials
Optical Properties of Materials
Nanomaterials
Nanobiotechnology
DOI - identifier 10.1038/s41598-017-15772-0
Copyright notice © 2017 The Author(s). Open Access This article is licensed under a Creative Commons Attribution 4.0
ISSN 2045-2322
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