A novel approach to controlling dissolved oxygen levels in laboratory experiments

Hassell, K, Coutin, P and Nugegoda, D 2009, 'A novel approach to controlling dissolved oxygen levels in laboratory experiments', Journal of Experimental Marine Biology and Ecology, vol. 371, no. 2, pp. 147-154.


Document type: Journal Article
Collection: Journal Articles

Title A novel approach to controlling dissolved oxygen levels in laboratory experiments
Author(s) Hassell, K
Coutin, P
Nugegoda, D
Year 2009
Journal name Journal of Experimental Marine Biology and Ecology
Volume number 371
Issue number 2
Start page 147
End page 154
Total pages 7
Publisher Elsevier Science Bv
Abstract Hypoxia is a widespread environmental stressor that affects marine, estuarine and freshwater systems worldwide. Investigating the effects of hypoxia on aquatic animals in the natural environment is difficult and expensive. Laboratory experiments provide an alternative that allows manipulation of environmental variables and simulation of altered conditions that are expected in the future. However, controlling dissolved oxygen (DO) levels precisely in laboratory test situations is challenging and generally costly and time intensive. In this paper, we describe a novel chamber design that is capable of maintaining low DO levels precisely for a period of at least 4 days with minimal re-adjustment and nitrogen gas requirement. The system is simple, inexpensive and easy to use, and offers the additional benefit of being portable. The utility of this design is demonstrated with an experiment to test the effects of hypoxia and high salinity on hatch rates and yolk-sac larvae survival in the estuarine fish black bream Acanthopagrus butcheri (Munro, 1949). The results show that early life-stage black bream are sensitive to hypoxia and increased salinity, indicating that deterioration in estuarine environmental conditions may have negative effects on spawning success and recruitment to fish populations. This new laboratory technique will be useful for testing future scenarios for the estuaries of southern Australia as predicted by climate change models. Other potential applications and modifications for further development of this new technique are discussed.
Subject Ecological Impacts of Climate Change
Keyword(s) black bream
climate change
embryo
hypoxia
salinity
sparidae
DOI - identifier 10.1016/j.jembe.2009.01.013
Copyright notice © 2009 Elsevier B.V. All rights reserved.
ISSN 0022-0981
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