An in-vitro upper gut simulator for assessing continuous gas production: A proof-of-concept using milk digestion

Pillai, N, Berean, K, Brkljaca, R, Greve, L, Kasapis, S and Kalantar Zadeh, K 2018, 'An in-vitro upper gut simulator for assessing continuous gas production: A proof-of-concept using milk digestion', Journal of Functional Foods, vol. 47, pp. 200-210.


Document type: Journal Article
Collection: Journal Articles

Title An in-vitro upper gut simulator for assessing continuous gas production: A proof-of-concept using milk digestion
Author(s) Pillai, N
Berean, K
Brkljaca, R
Greve, L
Kasapis, S
Kalantar Zadeh, K
Year 2018
Journal name Journal of Functional Foods
Volume number 47
Start page 200
End page 210
Total pages 11
Publisher Elsevier BV
Abstract Using gases as biomarkers is receiving increasing attention across the field of gut health. The use of in-vitro digestion systems that measure gas is common to understand some of the complex systems that create these gases and previously have been conducted using long time period sampling schemes which miss important signatures regarding the dynamics of gas production. Here the development of a mono-compartment in-vitro digestion simulator capable of recording vital dynamic information in real-time including: CO2 concentration; high sensitivity H2 concentration; and pH are presented and validated utilising milk. The impact of a simplified bacterial model, bacterial population size and the presence of lactase are investigated. The favourable gas production outcomes are obtained when lactase is present, at 10^6 CFU of bacteria, in good agreement with clinical observations. This proof-of-concept system demonstrates reliable and repeatable results and has the potential to enhance the information capacity of current and future in-vitro simulators.
Subject Electrical and Electronic Engineering not elsewhere classified
Nutrition and Dietetics not elsewhere classified
Keyword(s) CO2
Digestion simulator
Gas
H2
In-vitro
Milk
DOI - identifier 10.1016/j.jff.2018.05.054
Copyright notice © 2018 Elsevier
ISSN 1756-4646
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