A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics

Vemuri, R, Shinde, T, Shastri, M, Perera, A, Tristram, S, Martoni, C, Gundamaraju, R, Ahuja, K, Ball, M and Eri, R 2018, 'A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics', International Journal of Medical Sciences, vol. 15, no. 9, pp. 840-848.


Document type: Journal Article
Collection: Journal Articles

Title A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics
Author(s) Vemuri, R
Shinde, T
Shastri, M
Perera, A
Tristram, S
Martoni, C
Gundamaraju, R
Ahuja, K
Ball, M
Eri, R
Year 2018
Journal name International Journal of Medical Sciences
Volume number 15
Issue number 9
Start page 840
End page 848
Total pages 9
Publisher Ivyspring International Publisher
Abstract Background: The health benefits of probiotics are well established and known to be strain-specific. However, the role of probiotics obtained from different origins and their efficacy largely remains unexplored. The aim of this study is to investigate the in vitro efficacy of probiotics from different origins. Methods: Probiotic strains utilized in this study include Lactobacillus acidophilus DDS-1 (human origin), Bifidobacterium animalis ssp. lactis UABla-12 (human origin), L. plantarum UALp-05 (plant origin) and Streptococcus thermophilus UASt-09 (dairy origin). Screening assays such as in vitro digestion simulation, adhesion, cell viability and cytokine release were used to evaluate the probiotic potential. Results: All strains showed good resistance in the digestion simulation process, especially DDS-1 and UALp-05, which survived up to a range of 107 to 108 CFU/mL from an initial concentration of 109 CFU/mL. Two human colonic mucus-secreting cells, HT-29 and LS174T, were used to assess the adhesion capacity, cytotoxicity/viability, and cytokine quantification. All strains exhibited good adhesion capacity. No significant cellular cytotoxicity or loss in cell viability was observed. DDS-1 and UALp-05 significantly upregulated anti-inflammatory IL-10 and downregulated pro-inflammatory TNF-? cytokine production. All the strains were able to downregulate IL-8 cytokine levels. Conclusion: Of the 4 strains tested, DDS-1 demonstrated superior survival rates, good adhesion capacity and strong immunomodulatory effect under different experimental conditions.
Subject Gastroenterology and Hepatology
Nutritional Physiology
Keyword(s) Adhesion
Gastrointestinal survival
Immunomodulation
Probiotics
DOI - identifier 10.7150/ijms.25004
Copyright notice © Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/).
ISSN 1449-1907
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