Anti- HIV-1 activity of lactic acid in human cervicovaginal fluid

Tyssen, D, Wang, Y, Hayward, J and Gilda Tachedjian, et al., 2018, 'Anti- HIV-1 activity of lactic acid in human cervicovaginal fluid', mSphere, vol. 3, no. 4, pp. 1-18.


Document type: Journal Article
Collection: Journal Articles

Title Anti- HIV-1 activity of lactic acid in human cervicovaginal fluid
Author(s) Tyssen, D
Wang, Y
Hayward, J
Gilda Tachedjian, et al.,
Year 2018
Journal name mSphere
Volume number 3
Issue number 4
Start page 1
End page 18
Total pages 18
Publisher American Society for Microbiology
Abstract Women of reproductive age with a Lactobacillus-dominated vaginal microbiota have a reduced risk of acquiring and transmitting HIV and a vaginal pH of ~4 due to the presence of ~1% (wt/vol) lactic acid. While lactic acid has potent HIV virucidal activity in vitro, whether lactic acid present in the vaginal lumen inactivates HIV has not been investigated. Here we evaluated the anti-HIV-1 activity of native, minimally diluted cervicovaginal fluid obtained from women of reproductive age (n = 20) with vaginal microbiota dominated by Lactobacillus spp. Inhibition of HIVBa-L was significantly associated with the protonated form of lactic acid in cervicovaginal fluid. The HIVBa-L inhibitory activity observed in the < 3-kDa acidic filtrate was similar to that of the corresponding untreated native cervicovaginal fluid as well as that of clarified neat cervicovaginal fluid subjected to protease digestion. These ex vivo studies indicate that protonated lactic acid is a major anti-HIV-1 metabolite present in acidic cervicovaginal fluid, suggesting a potential role in reducing HIV transmission by inactivating virus introduced or shed into the cervicovaginal lumen.
Subject Microbiology not elsewhere classified
Keyword(s) HIV transmission
Human immunodeficiency virus
Lactobacillus
Metabolite
Microbiota
Vagina
Copyright notice © 2018 Tyssen et al.
ISSN 2379-5042
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